Academic Journal
Effect of MicroRNA-21 Transfection on In-vitro Differentiation of Human Naive CD4+ T Cells to Regulatory T Cells
| العنوان: | Effect of MicroRNA-21 Transfection on In-vitro Differentiation of Human Naive CD4+ T Cells to Regulatory T Cells |
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| المؤلفون: | Haideh Namdari, Mojgan Ghayedi, Jamshid Hadjati, Farhad Rezaei, Kurosh Kalantar, Parisa Rahimzadeh, Eisa Salehi |
| المصدر: | Iranian Journal of Allergy, Asthma and Immunology, Vol 16, Iss 3 (2017) |
| بيانات النشر: | Tehran University of Medical Sciences, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Foxp3, Mir-21, In-vitro differentiation, Naive CD4-positive T-lymphocytes, Regulatory T lymphocyte, Medicine |
| الوصف: | Regulatory T cells (Tregs) are important components of the immune system that modulate responses of other cells. These cells are involved in peripheral tolerance mechanisms, so defect in development and function of these cells can result in autoimmune disease. Increasing evidence supports the role of microRNAs-21 (miR-21) in the regulation of forkhead box P3 (Foxp3) expression in Tregs. We aimed to determine whether miR-21 transfection to naive CD4+ T cells can be useful in generation of iTregs in-vitro. We investigated in-vitro differentiation of miR-21-transfected naive CD4+ T cells to iTregs and compared these iTregs to cytokine-differentiated iTregs and control group. We showed that expression of Foxp3, transforming growth factor beta (TGF-β), and interleukin-10 (IL-10) are increased in iTregs generated after miR-21 transfection in comparison with cytokine-differentiated iTregs and control group. Our findings demonstrate that miR-21 has positive role in in-vitro generation of induced regulatory T-cells (iTregs). |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1735-1502 1735-5249 |
| Relation: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1047; https://doaj.org/toc/1735-1502; https://doaj.org/toc/1735-5249 |
| URL الوصول: | https://doaj.org/article/1c7f8002d2c14e6fbd3e48cc7245831e |
| رقم الانضمام: | edsdoj.1c7f8002d2c14e6fbd3e48cc7245831e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17351502 17355249 |
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