Academic Journal
Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy
| العنوان: | Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy |
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| المؤلفون: | Yi-Ju Chou, Ching-Cheng Lin, Ivan Dzhagalov, Nien-Jung Chen, Chao-Hsiung Lin, Chun-Cheng Lin, Szu-Ting Chen, Kuo-Hsin Chen, Shu-Ling Fu |
| المصدر: | Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) |
| بيانات النشر: | Nature Portfolio, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Abstract Toll-like receptors (TLRs) play crucial roles in host immune defenses. Recently, TLR-mediated autophagy is reported to promote immune responses via increasing antigen processing and presentation in antigen presenting cells. The present study examined whether the synthetic TLR4 activator (CCL-34) could induce autophagy to promote innate and adaptive immunity. In addition, the potential of CCL-34 as an immune adjuvant in vivo was also investigated. Our data using RAW264.7 cells and bone marrow-derived macrophages showed that CCL-34 induced autophagy through a TLR4-NF-κB pathway. The autophagy-related molecules (Nrf2, p62 and Beclin 1) were activated in RAW264.7 cells and bone marrow-derived macrophages under CCL-34 treatment. CCL-34-stimulated macrophages exhibited significant antigen-processing activity and induced the proliferation of antigen-specific CD4+T cells as well as the production of activated T cell-related cytokines, IL-2 and IFN-γ. Furthermore, CCL-34 immunization in mice induced infiltration of monocytes in the peritoneal cavity and elevation of antigen-specific IgG in the serum. CCL-34 treatment in vivo did not cause toxicity based on serum biochemical profiles. Notably, the antigen-specific responses induced by CCL-34 were attenuated by the autophagy inhibitor, 3-methyladenine. In summary, we demonstrated CCL-34 can induce autophagy to promote antigen-specific immune responses and act as an efficient adjuvant. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-020-65422-1 |
| URL الوصول: | https://doaj.org/article/1b2b59d6d750445faa8b12ddd17b55b0 |
| رقم الانضمام: | edsdoj.1b2b59d6d750445faa8b12ddd17b55b0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-020-65422-1 |