Academic Journal
Evaluation of cytokines in peripheral blood mononuclear cell supernatants for the diagnosis of tuberculosis
| العنوان: | Evaluation of cytokines in peripheral blood mononuclear cell supernatants for the diagnosis of tuberculosis |
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| المؤلفون: | Sariko M, Maro A, Gratz J, Houpt E, Kisonga R, Mpagama S, Heysell S, Mmbaga BT, Thomas TA |
| المصدر: | Journal of Inflammation Research, Vol Volume 12, Pp 15-22 (2018) |
| بيانات النشر: | Dove Medical Press, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Pathology LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | Cytokines, chemokines, Biomarkers, TB, and diagnostics, Pathology, RB1-214, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Margaretha Sariko,1–3 Athanasia Maro,1,3 Jean Gratz,1,4 Eric Houpt,4 Riziki Kisonga,1,5 Stellah Mpagama,1,5 Scott Heysell,4 Blandina T Mmbaga,1–3,* Tania A Thomas4,* 1Kilimanjaro Clinical Research Institute, Moshi, Tanzania; 2Kilimanjaro Christian Medical University College, Moshi, Tanzania; 3Kilimanjaro Christian Medical Centre, Moshi, Tanzania; 4Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, VA, USA; 5Kibong’oto Infectious Diseases Hospital, Kilimanjaro, Tanzania *These authors contributed equally to this work Introduction: There is active interest in leveraging host immune responses as biomarkers of tuberculosis (TB) disease activity. We had previously evaluated an immunodiagnostic test called the antibody in lymphocyte supernatant (ALS) assay. Here, we aimed to evaluate a panel of inflammatory mediators and associate the responses with the ALS results to identify a biosignature to distinguish TB cases from controls. Methodology: In this case–control study, adults with TB were compared to controls who were hospitalized for non-infectious conditions. Blood was collected at baseline and after 4 weeks of TB treatment (from TB cases only). Peripheral blood mononuclear cells were isolated and cultured without antigenic stimulation for 72 hours. Inflammatory mediators were measured using the Multiplex cytokine kit and compared between TB cases and controls; among TB cases, responses were compared over time. ALS and inflammatory mediator results were evaluated using generalized discriminant analysis to identify the optimal biosignature to predict TB. Results: When comparing inflammatory mediators between groups, IL-1ra, IL-1β, and granulocyte macrophage-colony stimulating factor (GM-CSF) were lower in TB cases (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1178-7031 |
| Relation: | https://www.dovepress.com/evaluation-of-cytokines-in-peripheral-blood-mononuclear-cell-supernata-peer-reviewed-article-JIR; https://doaj.org/toc/1178-7031 |
| URL الوصول: | https://doaj.org/article/19fd660240b94f758819f335aa2acc96 |
| رقم الانضمام: | edsdoj.19fd660240b94f758819f335aa2acc96 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11787031 |
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