Academic Journal
Genome Wide Analysis of the Transcriptional Profiles in Different Regions of the Developing Rice Grains
| العنوان: | Genome Wide Analysis of the Transcriptional Profiles in Different Regions of the Developing Rice Grains |
|---|---|
| المؤلفون: | Ting-Ying Wu, Marlen Müller, Wilhelm Gruissem, Navreet K. Bhullar |
| المصدر: | Rice, Vol 13, Iss 1, Pp 1-19 (2020) |
| بيانات النشر: | SpringerOpen, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Plant culture |
| مصطلحات موضوعية: | RNA-sequencing, Rice grain filling, Laser capture microdissection, Cross cells, Nucellar epidermis, Ovular vascular trace, Plant culture, SB1-1110 |
| الوصف: | Abstract Background Rice is an important food source for humans worldwide. Because of its nutritional and agricultural significance, a number of studies addressed various aspects of rice grain development and grain filling. Nevertheless, the molecular processes underlying grain filling and development, and in particular the contributions of different grain tissues to these processes, are not understood. Main Text Using RNA-sequencing, we profiled gene expression activity in grain tissues comprised of cross cells (CC), the nucellar epidermis (NE), ovular vascular trace (OVT), endosperm (EN) and the aleurone layer (AL). These tissues were dissected using laser capture microdissection (LCM) at three distinct grain development stages. The mRNA expression datasets offer comprehensive and new insights into the gene expression patterns in different rice grain tissues and their contributions to grain development. Comparative analysis of the different tissues revealed their similar and/or unique functions, as well as the spatio-temporal regulation of common and tissue-specific genes. The expression patterns of genes encoding hormones and transporters indicate an important role of the OVT tissue in metabolite transport during grain development. Gene co-expression network prediction on OVT-specific genes identified several distinct and common development-specific transcription factors. Further analysis of enriched DNA sequence motifs proximal to OVT-specific genes revealed known and novel DNA sequence motifs relevant to rice grain development. Conclusion Together, the dataset of gene expression in rice grain tissues is a novel and useful resource for further work to dissect the molecular and metabolic processes during rice grain development. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1939-8425 1939-8433 |
| Relation: | http://link.springer.com/article/10.1186/s12284-020-00421-4; https://doaj.org/toc/1939-8425; https://doaj.org/toc/1939-8433 |
| DOI: | 10.1186/s12284-020-00421-4 |
| URL الوصول: | https://doaj.org/article/1771f336a9de4a9b899d99160d3644fd |
| رقم الانضمام: | edsdoj.1771f336a9de4a9b899d99160d3644fd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19398425 19398433 |
|---|---|
| DOI: | 10.1186/s12284-020-00421-4 |