Academic Journal

Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis

التفاصيل البيبلوغرافية
العنوان: Tofu Wastewater Recovery for β-glucan Production by Pichia norvegensis and Candida tropicalis
المؤلفون: Deidita Nafisa Wahyudi, Gemilang Lara Utama, Andri Frediansyah
المصدر: Current Research in Green and Sustainable Chemistry, Vol 10, Iss , Pp 100445- (2025)
بيانات النشر: Elsevier, 2025.
سنة النشر: 2025
المجموعة: LCC:Chemistry
مصطلحات موضوعية: Pichia, Candida, β-glucan, FTIR, NMR, SEM, Chemistry, QD1-999
الوصف: This study aims to determine the applicability of tofu wastewater as a medium for yeast growth and to determine the optimal growth time of yeast to produce β-glucans, as well as the yield and characteristics of β-glucans extract. Pichia norvegensis and Candida tropicalis cell walls were extracted for β-glucan. FTIR, solid-state NMR, and SEM were utilized to investigate purity, structural differences, and antioxidant activity. Candida tropicalis (BCT) yielded 3.20 % crude β-glucan, while Pichia norvegiensis NYI (BPN) yielded 2.10 %. FTIR showed β-glucan impurities in mannoprotein-chitosan combinations. BPN and BCT have β-glucan bands at 1075, 1041, and 894 cm−1. The impurity of both samples was confirmed by NMR spectra, which showed that the broad signal of BPN and BCT chemical shift at 4.803–4.587 ppm and at 4.764–4.541 ppm, respectively, corresponded to l β-1,3-glucosidic and β-1,6-glucosidic linkage, with some unusual anomeric resonance at 5.094–5.024 ppm by SEM, BPN and BCT were porous, sheet-like, and fibrous. BPN had 20.07 % DPPH radical scavenging activity, while BCT had 36.01 %.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2666-0865
Relation: http://www.sciencedirect.com/science/article/pii/S2666086525000013; https://doaj.org/toc/2666-0865
DOI: 10.1016/j.crgsc.2025.100445
URL الوصول: https://doaj.org/article/ec15b1aa89354d36ace48be694291c80
رقم الانضمام: edsdoj.15b1aa89354d36ace48be694291c80
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:26660865
DOI:10.1016/j.crgsc.2025.100445