Academic Journal
Modeling population size independent tissue epigenomes by ChIL‐seq with single thin sections
| العنوان: | Modeling population size independent tissue epigenomes by ChIL‐seq with single thin sections |
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| المؤلفون: | Kazumitsu Maehara, Kosuke Tomimatsu, Akihito Harada, Kaori Tanaka, Shoko Sato, Megumi Fukuoka, Seiji Okada, Tetsuya Handa, Hitoshi Kurumizaka, Noriko Saitoh, Hiroshi Kimura, Yasuyuki Ohkawa |
| المصدر: | Molecular Systems Biology, Vol 17, Iss 11, Pp 1-20 (2021) |
| بيانات النشر: | Springer Nature, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) LCC:Medicine (General) |
| مصطلحات موضوعية: | dissociation‐free epigenome analysis, in situ epigenomics on single thin tissue section, tissue‐specific enhancers, transcriptional state decomposition, traveling ratio, Biology (General), QH301-705.5, Medicine (General), R5-920 |
| الوصف: | Abstract Recent advances in genome‐wide technologies have enabled analyses using small cell numbers of even single cells. However, obtaining tissue epigenomes with cell‐type resolution from large organs and tissues still remains challenging, especially when the available material is limited. Here, we present a ChIL‐based approach for analyzing the diverse cellular dynamics at the tissue level using high‐depth epigenomic data. “ChIL for tissues” allows the analysis of a single tissue section and can reproducibly generate epigenomic profiles from several tissue types, based on the distribution of target epigenomic states, tissue morphology, and number of cells. The proposed method enabled the independent evaluation of changes in cell populations and gene activation in cells from regenerating skeletal muscle tissues, using a statistical model of RNA polymerase II distribution on gene loci. Thus, the integrative analyses performed using ChIL can elucidate in vivo cell‐type dynamics of tissues. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1744-4292 |
| Relation: | https://doaj.org/toc/1744-4292 |
| DOI: | 10.15252/msb.202110323 |
| URL الوصول: | https://doaj.org/article/a14215197b00491ea6506a7f9089eef7 |
| رقم الانضمام: | edsdoj.14215197b00491ea6506a7f9089eef7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17444292 |
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| DOI: | 10.15252/msb.202110323 |