التفاصيل البيبلوغرافية
| العنوان: |
Protocol to study the direct binding of proteins to RNA:DNA hybrids or RNA-DNA chimeras in living cells using cross-linking immunoprecipitation |
| المؤلفون: |
Clara Bonnet, Ana Luisa Dian, Mélissa Leriche, Patricia Uguen, Stéphan Vagner |
| المصدر: |
STAR Protocols, Vol 5, Iss 3, Pp 103292- (2024) |
| بيانات النشر: |
Elsevier, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Science (General) |
| مصطلحات موضوعية: |
Cell Biology, Cell-based Assays, Molecular Biology, Science (General), Q1-390 |
| الوصف: |
Summary: RNA-binding proteins (RBPs) are involved in many biological processes. The direct interaction between protein and RNA can be studied using cross-linking immunoprecipitation (CLIP) techniques in living cells. Here, we present a protocol to characterize the direct binding of proteins to RNA:DNA hybrids or RNA-DNA chimeras in living cells using CLIP. We describe steps for RNA-protein UV-C cross-linking in living cells, isolating RNA-protein complexes, RNA labeling, and extracting nucleic acid. We then detail procedures for nuclease treatment and nucleic acid migration. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S266616672400457X; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2024.103292 |
| URL الوصول: |
https://doaj.org/article/1412586122da49d99bdad530739e8e21 |
| رقم الانضمام: |
edsdoj.1412586122da49d99bdad530739e8e21 |
| قاعدة البيانات: |
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