التفاصيل البيبلوغرافية
| العنوان: |
Spermiogenesis alterations in the absence of CTCF revealed by single cell RNA sequencing |
| المؤلفون: |
Ulises Torres-Flores, Fernanda Díaz-Espinosa, Tayde López-Santaella, Rosa Rebollar-Vega, Aarón Vázquez-Jiménez, Ian J. Taylor, Rosario Ortiz-Hernández, Olga M. Echeverría, Gerardo H. Vázquez-Nin, María Concepción Gutierrez-Ruiz, Inti Alberto De la Rosa-Velázquez, Osbaldo Resendis-Antonio, Abrahan Hernández-Hernandez |
| المصدر: |
Frontiers in Cell and Developmental Biology, Vol 11 (2023) |
| بيانات النشر: |
Frontiers Media S.A., 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
ScRNA-seq, mouse testis, spermatogenesis, CTCF, sperm, Biology (General), QH301-705.5 |
| الوصف: |
CTCF is an architectonic protein that organizes the genome inside the nucleus in almost all eukaryotic cells. There is evidence that CTCF plays a critical role during spermatogenesis as its depletion produces abnormal sperm and infertility. However, defects produced by its depletion throughout spermatogenesis have not been fully characterized. In this work, we performed single cell RNA sequencing in spermatogenic cells with and without CTCF. We uncovered defects in transcriptional programs that explain the severity of the damage in the produced sperm. In the early stages of spermatogenesis, transcriptional alterations are mild. As germ cells go through the specialization stage or spermiogenesis, transcriptional profiles become more altered. We found morphology defects in spermatids that support the alterations in their transcriptional profiles. Altogether, our study sheds light on the contribution of CTCF to the phenotype of male gametes and provides a fundamental description of its role at different stages of spermiogenesis. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2296-634X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fcell.2023.1119514/full; https://doaj.org/toc/2296-634X |
| DOI: |
10.3389/fcell.2023.1119514 |
| URL الوصول: |
https://doaj.org/article/1404543b20524ddaa6e5aadd64da58a8 |
| رقم الانضمام: |
edsdoj.1404543b20524ddaa6e5aadd64da58a8 |
| قاعدة البيانات: |
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