Academic Journal
An Improved Enzyme-Linked Immunosorbent Assay (ELISA) Based Protocol Using Seeds for Detection of Five Major Peanut Allergens Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8
| العنوان: | An Improved Enzyme-Linked Immunosorbent Assay (ELISA) Based Protocol Using Seeds for Detection of Five Major Peanut Allergens Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8 |
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| المؤلفون: | Arun K. Pandey, Rajeev K. Varshney, Hari K. Sudini, Manish K. Pandey |
| المصدر: | Frontiers in Nutrition, Vol 6 (2019) |
| بيانات النشر: | Frontiers Media S.A., 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Nutrition. Foods and food supply |
| مصطلحات موضوعية: | protocol, peanut allergens, Ara h 1, Ara h 2, Ara h 3, Ara h 6, Nutrition. Foods and food supply, TX341-641 |
| الوصف: | Peanut allergy is an important health concern among many individuals. As there is no effective treatment to peanut allergy, continuous monitoring of peanut-based products, and their sources is essential. Precise detection of peanut allergens is key for identification and development of improved peanut varieties with minimum or no allergens in addition to estimating the levels in peanut-based products available in food chain. The antibody based ELISA protocol along with sample preparation was standardized for Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8 to estimate their quantities in peanut seeds. Three different dilutions were optimized to precisely quantify target allergen proteins in peanut seeds such as Ara h 1 (1/1,000, 1/2,000, and 1/4,000), Ara h 2 and Ara h 3 (1/5,000, 1/10,000, and 1/20,000), Ara h 6 (1/40,000, 1/80,000, and 1/1,60,000), and Ara h 8 (1/10, 1/20, and 1/40). These dilutions were finalized for each allergen based on the accuracy of detection by achieving |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2296-861X |
| Relation: | https://www.frontiersin.org/article/10.3389/fnut.2019.00068/full; https://doaj.org/toc/2296-861X |
| DOI: | 10.3389/fnut.2019.00068 |
| URL الوصول: | https://doaj.org/article/139b360ff4d6477b90f1e230b7c74589 |
| رقم الانضمام: | edsdoj.139b360ff4d6477b90f1e230b7c74589 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2296861X |
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| DOI: | 10.3389/fnut.2019.00068 |