Academic Journal
Phenotypic and Genotypic Evaluation of Methicillin-resistant Staphylococcus aureus Strains Isolated from Children in Tehran Children\'s Medical Center, 2016
| العنوان: | Phenotypic and Genotypic Evaluation of Methicillin-resistant Staphylococcus aureus Strains Isolated from Children in Tehran Children\'s Medical Center, 2016 |
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| المؤلفون: | Shima Mahmoudi, Setareh Mamishi, Fatemeh Hasanvand, Mona Seif, Babak Pourakbari |
| المصدر: | Journal of Mazandaran University of Medical Sciences, Vol 29, Iss 177, Pp 30-41 (2019) |
| بيانات النشر: | Mazandaran University of Medical Sciences, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine LCC:Medicine (General) |
| مصطلحات موضوعية: | methicillin-resistant staphylococcus aureus, panton-valentine leukocidin, spa typing, sccmec typing, Medicine, Medicine (General), R5-920 |
| الوصف: | Background and purpose: The aims of this study were to evaluate the antibiotic resistance of Healthcare-associated Methicillin-resistant Staphylococcus aureus (HA-MRSA) and Community associated MRSA (CA-MRSA)-resistant strains of Staphylococcus aureus strains and to investigate the frequency of different types of spa typing, SCCmec I, II, III, IV, V, and type IV, among the strains of MRSA isolated from clinical specimens of children in Tehran Children's Medical Center Hospital. Materials and methods: In this cross-sectional study, antibiotic susceptibility test was performed using Kirby-Baer method according to the CLSI guidelines. Presence of mecA, PVL, spa genes, as well as different types of SCCmec I, II, III, IV, V, and major subtypes of SCCmec IV were investigated using Multiplex PCR. Results: Among 133 clinical isolates of S. aureus, 70 (53%) were methicillin-susceptible (MSSA) and 63 (47%) were MRSA. The mecA and PVL genes frequency distributions among MRSA strains were 100% and 3.1%, respectively. In the present study, there were 5 SCCmec (I-V) types, indicating wide variations in the hospital investigated. In addition, high prevalence of SCCmec III (30.1%) and III + Iva (23.8%) was observed. Of 63 strains of MRSA, 46 were classified into 11 spa types. The most common type was spa t037 that was found in 53.9% (n= 34) of the strains. Conclusion: High prevalence of SCCmec III + Iva and emergence of CA-MRSA strains in hospitals can be a serious warning to practitioners and infection control committees. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Persian |
| تدمد: | 1735-9260 1735-9279 |
| Relation: | http://jmums.mazums.ac.ir/article-1-13318-en.html; https://doaj.org/toc/1735-9260; https://doaj.org/toc/1735-9279 |
| URL الوصول: | https://doaj.org/article/133d7b08946546f1bb8030c209f76caa |
| رقم الانضمام: | edsdoj.133d7b08946546f1bb8030c209f76caa |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17359260 17359279 |
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