Academic Journal
Machine learning and genetic algorithm-guided directed evolution for the development of antimicrobial peptides
| العنوان: | Machine learning and genetic algorithm-guided directed evolution for the development of antimicrobial peptides |
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| المؤلفون: | Heqian Zhang, Yihan Wang, Yanran Zhu, Pengtao Huang, Qiandi Gao, Xiaojie Li, Zhaoying Chen, Yu Liu, Jiakun Jiang, Yuan Gao, Jiaquan Huang, Zhiwei Qin |
| المصدر: | Journal of Advanced Research, Vol 68, Iss , Pp 415-428 (2025) |
| بيانات النشر: | Elsevier, 2025. |
| سنة النشر: | 2025 |
| المجموعة: | LCC:Medicine (General) LCC:Science (General) |
| مصطلحات موضوعية: | Machine learning, Genetic algorithm, Directed evolution, Antimicrobial peptide, Medicine (General), R5-920, Science (General), Q1-390 |
| الوصف: | Introduction: Antimicrobial peptides (AMPs) are valuable alternatives to traditional antibiotics, possess a variety of potent biological activities and exhibit immunomodulatory effects that alleviate difficult-to-treat infections. Clarifying the structure-activity relationships of AMPs can direct the synthesis of desirable peptide therapeutics. Objectives: In this study, the lipopolysaccharide-binding domain (LBD) was identified through machine learning-guided directed evolution, which acts as a functional domain of the anti-lipopolysaccharide factor family of AMPs identified from Marsupenaeus japonicus. Methods: LBDA-D was identified as an output of this algorithm, in which the original LBDMj sequence was the input, and the three-dimensional solution structure of LBDB was determined using nuclear magnetic resonance. Furthermore, our study involved a comprehensive series of experiments, including morphological studies and in vitro and in vivo antibacterial tests. Results: The NMR solution structure showed that LBDB possesses a circular extended structure with a disulfide crosslink at the terminus and two 310-helices and exhibits a broad antimicrobial spectrum. In addition, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that LBDB induced the formation of a cluster of bacteria wrapped in a flexible coating that ruptured and consequently killed the bacteria. Finally, coinjection of LBDB, Vibrio alginolyticus and Staphylococcus aureus in vivo improved the survival of M. japonicus, demonstrating the promising therapeutic role of LBDB for treating infectious disease. Conclusions: The findings of this study pave the way for the rational drug design of activity-enhanced peptide antibiotics. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2090-1232 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S209012322400078X; https://doaj.org/toc/2090-1232 |
| DOI: | 10.1016/j.jare.2024.02.016 |
| URL الوصول: | https://doaj.org/article/1285232a15f847fab006ff21a96b712e |
| رقم الانضمام: | edsdoj.1285232a15f847fab006ff21a96b712e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20901232 |
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| DOI: | 10.1016/j.jare.2024.02.016 |