التفاصيل البيبلوغرافية
| العنوان: |
Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing |
| المؤلفون: |
Kei Takahashi, Shimpei I. Kubota, Shogo Ehata, Hiroki R. Ueda, Kohei Miyazono |
| المصدر: |
STAR Protocols, Vol 1, Iss 3, Pp 100191- (2020) |
| بيانات النشر: |
Elsevier, 2020. |
| سنة النشر: |
2020 |
| المجموعة: |
LCC:Science (General) |
| مصطلحات موضوعية: |
Cancer, Microscopy, Model Organisms, Science (General), Q1-390 |
| الوصف: |
Summary: Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry.For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017). |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166720301787; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2020.100191 |
| URL الوصول: |
https://doaj.org/article/d1252c164822453196fcd6ed38c58f8a |
| رقم الانضمام: |
edsdoj.1252c164822453196fcd6ed38c58f8a |
| قاعدة البيانات: |
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