التفاصيل البيبلوغرافية
| العنوان: |
Detection of N-glycolyl-neuraminic acid-containing glycolipids in human skin |
| المؤلفون: |
Michela Manni, Natalia Rodrigues Mantuano, Andreas Zingg, Elisabeth A. Kappos, Anna-Janina Behrens, Jonathan Back, Rainer Follador, Amir Faridmoayer, Heinz Läubli |
| المصدر: |
Frontiers in Immunology, Vol 14 (2023) |
| بيانات النشر: |
Frontiers Media S.A., 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: |
sialic acid, glycolipids, N-acetyl-neuraminic acid, N-glycolyl-neuraminic acid, cancer, Immunologic diseases. Allergy, RC581-607 |
| الوصف: |
Humans lack the enzyme that produces the sialic acid N-glycolyl neuraminic acid (Neu5Gc), but several lines of evidence have shown that Neu5Gc can be taken up by mammalian food sources and replace the common human sialic acid N-acetyl neuraminic acid (Neu5Ac) in glycans. Cancer tissue has been shown to have increased the presence of Neu5Gc and Neu5Gc-containing glycolipids such as the ganglioside GM3, which have been proposed as tumor-specific antigens for antibody treatment. Here, we show that a previously described antibody against Neu5Gc-GM3 is binding to Neu5GC-containing gangliosides and is strongly staining different cancer tissues. However, we also found a strong intracellular staining of keratinocytes of healthy skin. We confirmed this staining on freshly isolated keratinocytes by flow cytometry and detected Neu5Gc by mass spectrometry. This finding implicates that non-human Neu5Gc can be incorporated into gangliosides in human skin, and this should be taken into consideration when targeting Neu5Gc-containing gangliosides for cancer immunotherapy. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-3224 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fimmu.2023.1291292/full; https://doaj.org/toc/1664-3224 |
| DOI: |
10.3389/fimmu.2023.1291292 |
| URL الوصول: |
https://doaj.org/article/ad0da400a2bd4a93814c2f64ca1c03f6 |
| رقم الانضمام: |
edsdoj.0da400a2bd4a93814c2f64ca1c03f6 |
| قاعدة البيانات: |
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