التفاصيل البيبلوغرافية
| العنوان: |
Donepezil suppresses intracellular Ca2+ mobilization through the PI3K pathway in rodent microglia |
| المؤلفون: |
Yoshinori Haraguchi, Yoshito Mizoguchi, Masahiro Ohgidani, Yoshiomi Imamura, Toru Murakawa-Hirachi, Hiromi Nabeta, Hiroshi Tateishi, Takahiro A. Kato, Akira Monji |
| المصدر: |
Journal of Neuroinflammation, Vol 14, Iss 1, Pp 1-14 (2017) |
| بيانات النشر: |
BMC, 2017. |
| سنة النشر: |
2017 |
| المجموعة: |
LCC:Neurology. Diseases of the nervous system |
| مصطلحات موضوعية: |
Microglia, Calcium, Donepezil, Alzheimer’s disease, Phagocytosis, Nitric oxide, Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: |
Abstract Background Microglia are resident innate immune cells which release many factors including proinflammatory cytokines or nitric oxide (NO) when they are activated in response to immunological stimuli. Pathophysiology of Alzheimer’s disease (AD) is related to the inflammatory responses mediated by microglia. Intracellular Ca2+ signaling is important for microglial functions such as release of NO and cytokines. In addition, alteration of intracellular Ca2+ signaling underlies the pathophysiology of AD, while it remains unclear how donepezil, an acetylcholinesterase inhibitor, affects intracellular Ca2+ mobilization in microglial cells. Methods We examined whether pretreatment with donepezil affects the intracellular Ca2+ mobilization using fura-2 imaging and tested the effects of donepezil on phagocytic activity by phagocytosis assay in rodent microglial cells. Results In this study, we observed that pretreatment with donepezil suppressed the TNFα-induced sustained intracellular Ca2+ elevation in both rat HAPI and mouse primary microglial cells. On the other hand, pretreatment with donepezil did not suppress the mRNA expression of both TNFR1 and TNFR2 in rodent microglia we used. Pretreatment with acetylcholine but not donepezil suppressed the TNFα-induced intracellular Ca2+ elevation through the nicotinic α7 receptors. In addition, sigma 1 receptors were not involved in the donepezil-induced suppression of the TNFα-mediated intracellular Ca2+ elevation. Pretreatment with donepezil suppressed the TNFα-induced intracellular Ca2+ elevation through the PI3K pathway in rodent microglial cells. Using DAF-2 imaging, we also found that pretreatment with donepezil suppressed the production of NO induced by TNFα treatment and the PI3K pathway could be important for the donepezil-induced suppression of NO production in rodent microglial cells. Finally, phagocytosis assay showed that pretreatment with donepezil promoted phagocytic activity of rodent microglial cells through the PI3K but not MAPK/ERK pathway. Conclusions These suggest that donepezil could directly modulate the microglial function through the PI3K pathway in the rodent brain, which might be important to understand the effect of donepezil in the brain. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1742-2094 |
| Relation: |
http://link.springer.com/article/10.1186/s12974-017-1033-0; https://doaj.org/toc/1742-2094 |
| DOI: |
10.1186/s12974-017-1033-0 |
| URL الوصول: |
https://doaj.org/article/c0cbc32cede34161a6d1a156d20567fe |
| رقم الانضمام: |
edsdoj.0cbc32cede34161a6d1a156d20567fe |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder