Academic Journal
Interspecies Expression of an EF-HAND CALCIUM-BINDING PROTEIN in Xanthomonas perforans Leads to Reduced Virulence and Decreased Immune Evasion in Tomato Plants
| العنوان: | Interspecies Expression of an EF-HAND CALCIUM-BINDING PROTEIN in Xanthomonas perforans Leads to Reduced Virulence and Decreased Immune Evasion in Tomato Plants |
|---|---|
| المؤلفون: | Shaheen Bibi, Gerald V. Minsavage, J. Figueiredo, Sujan Timilsina, Kayla Margin, Juliana Quay, Hannah Bendure, Elizabeth Ryerson, Cliff Calloway, Jacob Andring, Aastha Subedi, Robert McKenna, Paul Gulig, Erica M. Goss, Jason C. Hurlbert, Jeffrey B. Jones |
| المصدر: | Molecular Plant-Microbe Interactions, Vol 37, Iss 11, Pp 784-800 (2024) |
| بيانات النشر: | The American Phytopathological Society, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Microbiology LCC:Botany |
| مصطلحات موضوعية: | calcium, hypersensitivity, tomato, type III secretion system (TTSS), Xanthomonas, Microbiology, QR1-502, Botany, QK1-989 |
| الوصف: | Many phytopathogenic bacteria require a type III secretion system (TTSS) to activate effector-triggered immunity (ETI). We identified a calcium-binding protein, EfhXXfa, in the citrus pathogen Xanthomonas citri subsp. aurantifolii that does not require a TTSS to activate reactive oxygen species (ROS) and elicit a hypersensitive reaction (HR) in tomato leaves following infection. Purified, recombinant EfhXXfa was shown to bind two moles of calcium per mole of protein, whereas mutation of the first of two EF-hands did not bind calcium. EfhXXfa expression was determined to be inducible in hrp-inducing medium. Additionally, growth of X. perforans transconjugants with and without the efhXXfa gene in hrp-inducing medium differed in intracellular calcium concentration; the transconjugant without efhXXfa yielded higher cell pellet masses and higher increased intracellular calcium concentrations relative to cells expressing EfhXXfa. An EfhXXfa homolog, EfhXXe, present in the pepper pathogen X. euvesicatoria, triggered ROS production and an HR in tomato leaves when expressed in the tomato pathogen X. perforans and is a host-limiting factor. Interestingly, all tested X. perforans and X. euvesicatoria strains pathogenic on tomato contain a stop codon immediately upstream of the first EF-hand domain in the efhXXe gene, whereas most X. euvesicatoria strains pathogenic on pepper do not. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1943-7706 0894-0282 |
| Relation: | https://doaj.org/toc/0894-0282; https://doaj.org/toc/1943-7706 |
| DOI: | 10.1094/MPMI-07-24-0073-R |
| URL الوصول: | https://doaj.org/article/0be00239cfaa489fad62e5a14a3eb7cc |
| رقم الانضمام: | edsdoj.0be00239cfaa489fad62e5a14a3eb7cc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19437706 08940282 |
|---|---|
| DOI: | 10.1094/MPMI-07-24-0073-R |