Academic Journal
DNA Fingerprinting of Olive Varieties by Microsatellite Markers
| العنوان: | DNA Fingerprinting of Olive Varieties by Microsatellite Markers |
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| المؤلفون: | Dunja Bandelj, Jernej Jakše, Branka Javornik |
| المصدر: | Food Technology and Biotechnology, Vol 40, Iss 3, Pp 185-190 (2002) |
| بيانات النشر: | University of Zagreb Faculty of Food Technology and Biotechnology, 2002. |
| سنة النشر: | 2002 |
| المجموعة: | LCC:Biotechnology LCC:Food processing and manufacture |
| مصطلحات موضوعية: | olives (Olea europaea L.), microsatellites, varietal identification, Biotechnology, TP248.13-248.65, Food processing and manufacture, TP368-456 |
| الوصف: | Microsatellites combine several features of an ultimate molecular marker and they are used increasingly in various plant genetic studies and applications. In this work we report on the utilisation of fourteen previously developed olive microsatellite markers for the identification and differentiation of a set of nineteen olive varieties. All analysed microsatellite markers revealed a high level of polymorphism that allowed unique genotyping of the examined varieties. Ninety-six alleles were detected at all 14 loci, which multiplied into a large number of observed genotypes, giving high discrimination value for varietal identification. A minimum number of three microsatellite markers was chosen for the rapid and unambiguous varietal identification of nineteen olive varieties and only two markers were sufficient for differentiation of five local varieties. DNA fingerprints of olive cultivars by means of microsatellites provided meaningful data, which can be extended by additional olive varieties or new microsatellites and used for accurate inter-laboratory comparison. The data obtained can be used for the varietal survey and construction of a database of all olive varieties grown in Slovenia providing also additional genetic information on the agronomic and quality characteristics of the olive varieties. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1330-9862 1334-2606 |
| Relation: | http://hrcak.srce.hr/file/263047; https://doaj.org/toc/1330-9862; https://doaj.org/toc/1334-2606 |
| URL الوصول: | https://doaj.org/article/0b9b8bf0b2fd4f0290c25d82e2d014bd |
| رقم الانضمام: | edsdoj.0b9b8bf0b2fd4f0290c25d82e2d014bd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13309862 13342606 |
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