Academic Journal
IbpB‐bound substrate release in living cells as revealed by unnatural amino acid‐mediated photo‐crosslinking
| العنوان: | IbpB‐bound substrate release in living cells as revealed by unnatural amino acid‐mediated photo‐crosslinking |
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| المؤلفون: | Xiaodong Shi, Anastasia N. Ezemaduka |
| المصدر: | FEBS Open Bio, Vol 10, Iss 10, Pp 2081-2088 (2020) |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | IbpB, photo‐crosslinking, small heat shock proteins, substrate binding, unnatural amino acid, Biology (General), QH301-705.5 |
| الوصف: | Small heat shock proteins (sHSPs) are known to bind non‐native substrates and prevent irreversible aggregation in an ATP‐independent manner. However, the dynamic interaction between sHSPs and their substrates in vivo is less studied. Here, by utilizing a genetically incorporated crosslinker, we characterized the interaction between sHSP IbpB and its endogenous substrates in living cells. Through photo‐crosslinking analysis of five Bpa variants of IbpB, we found that the substrate binding of IbpB in living cells is reversible upon short‐time exposure at 50 °C. Our data provide in vivo evidence that IbpB engages in dynamic substrate release under nonstress conditions and suggest that photo‐crosslinking may be a suitable method for investigating dynamic interaction between molecular chaperones and their substrates in living cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2211-5463 74877852 |
| Relation: | https://doaj.org/toc/2211-5463 |
| DOI: | 10.1002/2211-5463.12957 |
| URL الوصول: | https://doaj.org/article/ad0b7f7180b74877852eba332f9d47f6 |
| رقم الانضمام: | edsdoj.0b7f7180b74877852eba332f9d47f6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22115463 74877852 |
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| DOI: | 10.1002/2211-5463.12957 |