Academic Journal
Topical neurokinin-1 receptor antagonism ameliorates ocular pain and prevents corneal nerve degeneration in an animal model of dry eye disease
| العنوان: | Topical neurokinin-1 receptor antagonism ameliorates ocular pain and prevents corneal nerve degeneration in an animal model of dry eye disease |
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| المؤلفون: | Amirreza Naderi, Yukako Taketani, Shudan Wang, Francesca Kahale, Ann Yung, Pier Luigi Surico, Yihe Chen, Reza Dana |
| المصدر: | PAIN Reports, Vol 10, Iss 1, p e1232 (2025) |
| بيانات النشر: | Wolters Kluwer, 2025. |
| سنة النشر: | 2025 |
| المجموعة: | LCC:Anesthesiology |
| مصطلحات موضوعية: | Anesthesiology, RD78.3-87.3 |
| الوصف: | Abstract. Introduction:. Ocular pain is a common complaint to eye care providers, associated with a variety of ocular conditions, among which dry eye disease (DED) is affecting millions of people worldwide. Despite being highly prevalent, ocular pain is not managed adequately in the clinic. Objectives:. The aim of this study was to investigate the analgesic potential of neurokinin-1 receptor (NK1R) antagonism in DED. Methods:. Dry eye disease was induced in mice, and an NK1R antagonist L-733,060 was topically administered twice daily throughout the study for 14 days. Hyperalgesia and allodynia were assessed using the eye-wiping test and palpebral ratio measurements. Corneas were collected for measuring substance P (SP) levels by enzyme-linked immunosorbent assay (ELISA) and imaging nerves by immunostaining. Trigeminal ganglions (TG) were collected to determine SP levels by ELISA and transient receptor potential cation channel subfamily V member 1 (TRPV1), transient receptor potential cation channel subfamily M (melastatin) member 8, c-Fos, and activating transcription factor 3 (ATF3) mRNA levels by real-time polymerase chain reaction. Results:. Treating DED mice with L-733,060 resulted in a significant reduction in eye wipe behavior, a significant increase in palpebral ratio, and significant decreases in SP levels in both the cornea and TG compared with the vehicle-treated group. In addition, NK1R antagonist treatment significantly suppressed the upregulation of TRPV1, ATF3, and c-Fos and prevented corneal nerve loss. Conclusion:. Neurokinin-1 receptor antagonism effectively reduced ocular nociception, decreased neuronal activation, and preserved corneal nerves in mice with DED. These findings suggest that blockade of SP signaling pathway is a promising therapeutic strategy for managing DED pain. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2471-2531 00000000 |
| Relation: | http://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000001232; https://doaj.org/toc/2471-2531 |
| DOI: | 10.1097/PR9.0000000000001232 |
| URL الوصول: | https://doaj.org/article/0b52535f226445078e9486df3cd4010f |
| رقم الانضمام: | edsdoj.0b52535f226445078e9486df3cd4010f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24712531 00000000 |
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| DOI: | 10.1097/PR9.0000000000001232 |