التفاصيل البيبلوغرافية
| العنوان: |
Single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells |
| المؤلفون: |
Laura Mincarelli, Vladimir Uzun, David Wright, Anita Scoones, Stuart A. Rushworth, Wilfried Haerty, Iain C. Macaulay |
| المصدر: |
Communications Biology, Vol 6, Iss 1, Pp 1-11 (2023) |
| بيانات النشر: |
Nature Portfolio, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
Biology (General), QH301-705.5 |
| الوصف: |
Abstract Single-cell approaches have revealed that the haematopoietic hierarchy is a continuum of differentiation, from stem cell to committed progenitor, marked by changes in gene expression. However, many of these approaches neglect isoform-level information and thus do not capture the extent of alternative splicing within the system. Here, we present an integrated short- and long-read single-cell RNA-seq analysis of haematopoietic stem and progenitor cells. We demonstrate that over half of genes detected in standard short-read single-cell analyses are expressed as multiple, often functionally distinct, isoforms, including many transcription factors and key cytokine receptors. We observe global and HSC-specific changes in gene expression with ageing but limited impact of ageing on isoform usage. Integrating single-cell and cell-type-specific isoform landscape in haematopoiesis thus provides a new reference for comprehensive molecular profiling of heterogeneous tissues, as well as novel insights into transcriptional complexity, cell-type-specific splicing events and consequences of ageing. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2399-3642 |
| Relation: |
https://doaj.org/toc/2399-3642 |
| DOI: |
10.1038/s42003-023-04936-6 |
| URL الوصول: |
https://doaj.org/article/0a5d14d83c0d45119b67eeb0035dc63b |
| رقم الانضمام: |
edsdoj.0a5d14d83c0d45119b67eeb0035dc63b |
| قاعدة البيانات: |
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