Academic Journal
EPIDIDYMAL SPERMATOZOA QUALITY OF ETAWA CROSSBREED GOAT IN TRIS EXTENDER SUPPLEMENTED WITH VARIOUS LACTOSE CONCENTRATIONS
| العنوان: | EPIDIDYMAL SPERMATOZOA QUALITY OF ETAWA CROSSBREED GOAT IN TRIS EXTENDER SUPPLEMENTED WITH VARIOUS LACTOSE CONCENTRATIONS |
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| المؤلفون: | Muhammad Riyadhi, Agus Setiawan, Herdis Herdis, Muhammad Rizal |
| المصدر: | Jurnal Kedokteran Hewan, Vol 11, Iss 1, Pp 15-18 (2017) |
| بيانات النشر: | Universitas Syiah Kuala, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | epididymal spermatozoa, etawa crossbreed, lactose, preservation, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | This research was conducted to investigate the effect of various concentrations of lactose supplementation in Tris extender for maintaining the quality of Etawa crossbreed goat epididymal spermatozoa stored at 3-5° C. Semen in the control group was diluted with a tris extender containing 20% egg yolk without lactose. Semen in the test groups was diluted with a tris extender containing 20% egg yolk and added with 0.3% (0.3 g per 100 mL extender) and 0.6% lactose for group TL1 and TL2, respectively. Parameters evaluated of the fresh epididymal spermatozoa were motility, concentration, percentage of live, and abnormality of spermatozoa, while for diluted-spermatozoa were motility and percentage of live spermatozoa. Spermatozoa observation was conducted until it reaches 40% motility. The results showed that the mean percentage of motility, live sperm, concentration, and abnormality of epididymal spermatozoa were 70%; 81%; 3,220x106 cells/mL; and 4.30%, respectively in all group. After dilution, the percentage of motility and live spermatozoa were also 70% and 81.00±1.58%, respectively in all groups. The decreasing of spermatozoa motility was observed on day 4 of storage, in which percentage of spermatozoa motility in control group (40.00±0.00%) was significantly lower (P0.05) than those in TL1 (44.00±2.24%) and TL2(45.00±0.00%) groups. Percentage of live spermatozoa in control (63.20±2.68%) was not significantly different (P0.05) than TL1(65.40±1.95%) and TL2 (65.60±1.95%). In conclusion, the supplementation of lactose into Tris extender could maintain the epididymal spermatozoa of Etawa crossbreed for 3 days of storage at 3-5° C. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Indonesian |
| تدمد: | 1978-225X 2502-5600 |
| Relation: | https://jurnal.usk.ac.id/JKH/article/view/5136; https://doaj.org/toc/1978-225X; https://doaj.org/toc/2502-5600 |
| DOI: | 10.21157/j.ked.hewan.v11i1.5136 |
| URL الوصول: | https://doaj.org/article/0a5ae6731ad94fc38eab4b883444cd4a |
| رقم الانضمام: | edsdoj.0a5ae6731ad94fc38eab4b883444cd4a |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 1978225X 25025600 |
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| DOI: | 10.21157/j.ked.hewan.v11i1.5136 |