Academic Journal
In vitro propagation of Caesalpinia spinosa (Mol.) O. Kuntz from axillary buds of selected trees
| العنوان: | In vitro propagation of Caesalpinia spinosa (Mol.) O. Kuntz from axillary buds of selected trees |
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| المؤلفون: | Jenny E Núñez Núñez, Elisa Quiala, Manuel de Feria, Saúl Mestanza, Silvana Teanga |
| المصدر: | Biotecnología Vegetal, Vol 17, Iss 2 (2017) |
| بيانات النشر: | Universidad Central Marta Abreu de Las Villas, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Agriculture LCC:Plant culture |
| مصطلحات موضوعية: | Agriculture, Plant culture, SB1-1110 |
| الوصف: | Guarango or tara [Caesalpinia spinosa (Mol.) O. Kuntz] is a tree native to the Andes, with great economic importance and for reforestation programs. The aim of this work was to in vitro propagate this specie from axillary buds of selected trees. During in vitro establishment, the effect of sodium hypochlorite (3.0%) with different times of disinfection (5.0, 10, 15 min), as well as the effect of 6-BAP on the in vitro response of buds were studied. For multiplication, different combination of 6-BAP with 0.1 mg l-1 ANA were tested. A free-growth regulator culture medium was used for rooting. The best results for in vitro establishment were achieved with a disinfection treatment with sodium hypochlorite 3.0% for 10 minutes and cultivation in a culture medium with 0.25 mg l-1 6-BAP, which 90% of buds in vitro established, with a length of 6.71 cm. The highest multiplication rate of shoot (2.88 per explant) was obtained with 1.0 mg l-1 6-BAP and 0.1 mg l-1 ANA, after 60 days of culture. The 55% of these shoots developed roots in a half-strength basal salts MS culture medium free of regulators of growth. Keywords: biodiversity, conservation, forest plant, guarango, tissue culture |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Spanish; Castilian |
| تدمد: | 1609-1841 2074-8647 |
| Relation: | https://revista.ibp.co.cu/index.php/BV/article/view/543; https://doaj.org/toc/1609-1841; https://doaj.org/toc/2074-8647 |
| URL الوصول: | https://doaj.org/article/09ef56484bfa42df8af1e8c590f9d883 |
| رقم الانضمام: | edsdoj.09ef56484bfa42df8af1e8c590f9d883 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16091841 20748647 |
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