Academic Journal
In situ transplantation of adipose-derived stem cells via photoactivation improves glucose metabolism in obese mice
| العنوان: | In situ transplantation of adipose-derived stem cells via photoactivation improves glucose metabolism in obese mice |
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| المؤلفون: | Luochen Zhu, Ziqian Feng, Xin Shu, Qian Gao, Jiaqi Wu, Zuoqin Du, Rong Li, Liqun Wang, Ni Chen, Yi Li, Mao Luo, Jianbo Wu |
| المصدر: | Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-13 (2021) |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Photoactivation, Adipose tissue derived stem cells, Implantation, Insulin resistance, High-fat diet, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Accumulating evidence suggests that enhanced adipose tissue macrophages (ATMs) are associated with metabolic disorders in obesity and type 2 diabetes. However, therapeutic persistence and reduced homing stem cell function following cell delivery remains a critical hurdle for the clinical translation of stem cells in current approaches. Methods We demonstrate that the effect of a combined application of photoactivation and adipose-derived stem cells (ASCs) using transplantation into visceral epididymal adipose tissue (EAT) in obesity. Cultured ASCs were derived from subcutaneous white adipose tissue isolated from mice fed a normal diet (ND). Results In diet-induced obesity, implantation of light-treated ASCs improved glucose tolerance and ameliorated systemic insulin resistance. Intriguingly, compared with non-light-treated ASCs, light-treated ASCs reduced monocyte infiltration and the levels of ATMs in EAT. Moreover, implantation of light-treated ASCs exerts more anti-inflammatory effects by suppressing M1 polarization and enhancing macrophage M2 polarization in EAT. Mass spectrometry revealed that light-treated human obese ASCs conditioned medium retained a more complete secretome with significant downregulation of pro-inflammatory cytokines and chemokines. Conclusions These data suggest that the combined application of photoactivation and ASCs using transplantation into dysfunctional adipose tissue contribute to selective suppression of inflammatory responses and protection from insulin resistance in obesity and type 2 diabetes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| Relation: | https://doaj.org/toc/1757-6512 |
| DOI: | 10.1186/s13287-021-02494-4 |
| URL الوصول: | https://doaj.org/article/095e1301631e49b5aa9d9499d6d86612 |
| رقم الانضمام: | edsdoj.095e1301631e49b5aa9d9499d6d86612 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-021-02494-4 |