Academic Journal
Protein tyrosine phosphatase PTPL1 suppresses lung cancer through Src/ERK/YAP1 signaling
| العنوان: | Protein tyrosine phosphatase PTPL1 suppresses lung cancer through Src/ERK/YAP1 signaling |
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| المؤلفون: | Jing Wang, Shuanghui Li, Xiujuan Zhang, Ning Zhu, Ruzetuoheti Yiminniyaze, Liang Dong, Chengwei Li, Wumaier Gulinuer, Jingwen Xia, Jing Li, Daibing Zhou, Xinning Liu, Youzhi Zhang, Yuanyuan Zhang, Shengqing Li |
| المصدر: | Thoracic Cancer, Vol 13, Iss 21, Pp 3042-3051 (2022) |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | non‐small cell lung cancer, proliferation, PTPL1, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Abstract Background To reveal the function of protein tyrosine phosphatase‐L1 (PTPL1) in lung adenocarcinoma. Methods Lung cancer cell lines were transfected with short hairpin RNA against PTPL1 (shPTPL1 group) or negative control (shmock group). Quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blotting were used to verify the transfection efficacy. Cell proliferation was analyzed by ethynyldeoxyuridine (EdU), Cell counting kit 8 (CCK8), and colony formation assay after PTPL1 or PTPL1 and yes‐associated protein (YAP1) knockdown. The effect of PTPL1 on tumor growth was examined in a xenograft lung cancer model. Results PTPL1 was downregulated in various types of lung cancer cell lines. The EdU, CCK8, colony formation assays and investigation using a xenograft lung cancer model indicated that PTPL1 knockdown increased the proliferation of lung cancer cells. Mechanistically, PTPL1 knockdown induced the activation of the Proto‐oncogene tyrosine‐protein kinase SRC (Src)/Extracellular regulated MAP kinase (ERK) pathway and thereby promoted yes‐associated protein (YAP1) nuclear translocation and activation. Conclusions In our study, PTPL1 played a crucial suppressive role in the pathogenesis of lung cancer potentially through counteracting the Src/ERK/YAP1 pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1759-7714 1759-7706 |
| Relation: | https://doaj.org/toc/1759-7706; https://doaj.org/toc/1759-7714 |
| DOI: | 10.1111/1759-7714.14657 |
| URL الوصول: | https://doaj.org/article/08e5311bfd30426194785a95a0b21c55 |
| رقم الانضمام: | edsdoj.08e5311bfd30426194785a95a0b21c55 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17597714 17597706 |
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| DOI: | 10.1111/1759-7714.14657 |