التفاصيل البيبلوغرافية
| العنوان: |
Comparative Genome Analysis Reveals Cis-Regulatory Elements on Gene-Sized Chromosomes of Ciliated Protists |
| المؤلفون: |
Weibo Zheng, Huan Dou, Chao Li, Saleh A. Al-Farraj, Adam Byerly, Naomi A. Stover, Weibo Song, Xiao Chen, Lifang Li |
| المصدر: |
Frontiers in Microbiology, Vol 13 (2022) |
| بيانات النشر: |
Frontiers Media S.A., 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
ciliates, Pseudokeronopsis carnea, Pseudokeronopsis flava, phylogenomics, nanochromosome, Microbiology, QR1-502 |
| الوصف: |
Gene-sized chromosomes are a distinct feature of the macronuclear genome in ciliated protists known as spirotrichs. These nanochromosomes are often only several kilobase pairs long and contain a coding region for a single gene. However, the ways in which transcription is regulated on nanochromosomes is still largely unknown. Here, we generated macronuclear genome assemblies for two species of Pseudokeronopsis ciliates to better understand transcription regulation on gene-sized chromosomes. We searched within the short subtelomeric regions for potential cis-regulatory elements and identified distinct AT-rich sequences conserved in both species, at both the 5’ and 3’ end of each gene. We further acquired transcriptomic data for these species, which showed the 5’ cis-regulatory element is associated with active gene expression. Gene family evolution analysis suggests nanochromosomes in spirotrichs may originated approximately 900 million years ago. Together our comparative genomic analyses reveal novel insights into the biological roles of cis-regulatory elements on gene-sized chromosomes. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-302X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmicb.2022.775646/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2022.775646 |
| URL الوصول: |
https://doaj.org/article/0844cdcf78fe4db7b28f307155055717 |
| رقم الانضمام: |
edsdoj.0844cdcf78fe4db7b28f307155055717 |
| قاعدة البيانات: |
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