Academic Journal

Genetic diversity, population structure, and relationships in a collection of pepper (Capsicum spp.) landraces from the Spanish centre of diversity revealed by genotyping-by-sequencing (GBS)

التفاصيل البيبلوغرافية
العنوان: Genetic diversity, population structure, and relationships in a collection of pepper (Capsicum spp.) landraces from the Spanish centre of diversity revealed by genotyping-by-sequencing (GBS)
المؤلفون: Leandro Pereira-Dias, Santiago Vilanova, Ana Fita, Jaime Prohens, Adrián Rodríguez-Burruezo
المصدر: Horticulture Research, Vol 6, Iss 1, Pp 1-13 (2019)
بيانات النشر: Oxford University Press, 2019.
سنة النشر: 2019
المجموعة: LCC:Botany
LCC:Plant culture
مصطلحات موضوعية: Botany, QK1-989, Plant culture, SB1-1110
الوصف: Abstract Pepper (Capsicum spp.) is one of the most important vegetable crops; however, pepper genomic studies lag behind those of other important Solanaceae. Here we present the results of a high-throughput genotyping-by-sequencing (GBS) study of a collection of 190 Capsicum spp. accessions, including 183 of five cultivated species (C. annuum, C. chinense, C. frutescens, C. baccatum, and C. pubescens) and seven of the wild form C. annuum var. glabriusculum. Sequencing generated 6,766,231 high-quality read tags, of which 40.7% were successfully aligned to the reference genome. SNP calling yielded 4083 highly informative segregating SNPs. Genetic diversity and relationships of a subset of 148 accessions, of which a complete passport information was available, was studied using principal components analysis (PCA), discriminant analysis of principal components (DAPC), and phylogeny approaches. C. annuum, C. baccatum, and C. chinense were successfully separated by all methods. Our population was divided into seven clusters by DAPC, where C. frutescens accessions were clustered together with C. chinense. C. annuum var. glabriusculum accessions were spread into two distinct genetic pools, while European accessions were admixed and closely related. Separation of accessions was mainly associated to differences in fruit characteristics and origin. Phylogeny studies showed a close relation between Spanish and Mexican accessions, supporting the hypothesis that the first arose from a main genetic flow from the latter. Tajima’s D statistic values were consistent with positive selection in the C. annuum clusters, possibly related to domestication or selection towards traits of interest. This work provides comprehensive and relevant information on the origin and relationships of Spanish landraces and for future association mapping studies in pepper.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2052-7276
Relation: https://doaj.org/toc/2052-7276
DOI: 10.1038/s41438-019-0132-8
URL الوصول: https://doaj.org/article/052e4b20745b4cbba37a9a8cd3fb3ae3
رقم الانضمام: edsdoj.052e4b20745b4cbba37a9a8cd3fb3ae3
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20527276
DOI:10.1038/s41438-019-0132-8