Academic Journal
ANALYSIS OF MUTATIONS OF TUBERCULOUS MYCOBACTERIA DEFINING DRUG RESISTANCE IN HIV POSITIVE AND HIV NEGATIVE TUBERCULOSIS PATIENTS WITHOUT PRIOR HISTORY OF TREATMENT IN SVERDLOVSK REGION
| العنوان: | ANALYSIS OF MUTATIONS OF TUBERCULOUS MYCOBACTERIA DEFINING DRUG RESISTANCE IN HIV POSITIVE AND HIV NEGATIVE TUBERCULOSIS PATIENTS WITHOUT PRIOR HISTORY OF TREATMENT IN SVERDLOVSK REGION |
|---|---|
| المؤلفون: | G. V. Panov, S. N. Andreevskaya, E. E. Larionova, A. I. Tsvetkov, L. N. Chernousova |
| المصدر: | Туберкулез и болезни лёгких, Vol 95, Iss 2, Pp 27-32 (2017) |
| بيانات النشر: | New Terra Publishing House, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Diseases of the respiratory system |
| مصطلحات موضوعية: | микобактерии туберкулеза, туберкулез, ассоциированный с вич, лекарственная устойчивость, биологические микрочипы, спектр мутаций, Diseases of the respiratory system, RC705-779 |
| الوصف: | Goal of the study: to identify profile of mutations of tuberculous mycobacteria responsible for resistance to anti-tuberculosis drugs in HIV positive and HIV negative tuberculosis patients without prior history of treatment.Materials and methods. 165 strains of tuberculous mycobacteria from HIV positive patients and 166 strains of tuberculous mycobacteria from HIV negative patients were studied in Sverdlovsk Region (TB Dispensary, Yekaterinburg). Mutations in genes were identified using microchips of TB-BIOCHIP® and TB-BIOCHIP®-2 in compliance with the manufacturer's guidelines (OOO Biochip-IMB, Moscow).Results. It was observed that 85/165 (51.52%) strains isolated from HIV positive tuberculosis patients and 58/166 (34.94%) strains isolated from tuberculosis patients not associated with HIV possessed MDR genotype (p < 0.01). The majority of MDR strains had mutations in the 531th codon of rpoB (Ser→Leu) and 315th codon of katG (Ser→Thr) (64/85, 75.29% and 38/58, 65.52% respective the groups), resulting in the high level of resistance to rifampicin and isoniazid. Each group also had approximately equal ratio (11/165, 6.67% and 12/166, 7.23% respective the groups) of strains with genomic mutations defining the resistance to isoniazid, rifampicin and fluoruquinolones. No confident difference was found in mutation patterns of genome of tuberculous mycobacteria isolated from HIV positive and HIV negative tuberculosis patients. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Russian |
| تدمد: | 2075-1230 2542-1506 02962160 |
| Relation: | https://www.tibl-journal.com/jour/article/view/972; https://doaj.org/toc/2075-1230; https://doaj.org/toc/2542-1506 |
| DOI: | 10.21292/2075-1230-2017-95-2-27-32 |
| URL الوصول: | https://doaj.org/article/02962160cc1e4891a0afd5c0e0fc414d |
| رقم الانضمام: | edsdoj.02962160cc1e4891a0afd5c0e0fc414d |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 20751230 25421506 02962160 |
|---|---|
| DOI: | 10.21292/2075-1230-2017-95-2-27-32 |