التفاصيل البيبلوغرافية
| العنوان: |
Development of an Integrated CMUTs-Based Resonant Biosensor for Label-Free Detection of DNA with Improved Selectivity by Ethylene-Glycol Alkanethiols |
| المؤلفون: |
Zhikang Li, Yihe Zhao, Gian Luca Barbruni, Jie Li, Zixuan Li, Jiawei Yuan, Ping Yang, Libo Zhao, Zhuangde Jiang, Sandro Carrara |
| المصدر: |
Engineering, Vol 41, Iss , Pp 231-241 (2024) |
| بيانات النشر: |
Elsevier, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Engineering (General). Civil engineering (General) |
| مصطلحات موضوعية: |
Capacitive micromachined ultrasonic transducers (CMUTs), DNA detection, Self-assembled monolayer (SAM), Ethylene-glycol alkanethiols, Application-specific integrated circuit (ASIC), Engineering (General). Civil engineering (General), TA1-2040 |
| الوصف: |
Gravimetric resonant-inspired biosensors have attracted increasing attention in industrial and point-of-care applications, enabling label-free detection of biomarkers such as DNA and antibodies. Capacitive micromachined ultrasonic transducers (CMUTs) are promising tools for developing miniaturized high-performance biosensing complementary metal–oxide–silicon (CMOS) platforms. However, their operability is limited by inefficient functionalization, aggregation, crosstalk in the buffer, and the requirement for an external high-voltage (HV) power supply. In this study, we aimed to propose a CMUTs-based resonant biosensor integrated with a CMOS front–end interface coupled with ethylene–glycol alkanethiols to detect single-stranded DNA oligonucleotides with large specificity. The topography of the functionalized surface was characterized by energy-dispersive X-ray microanalysis. Improved selectivity for on-chip hybridization was demonstrated by comparing complementary and non-complementary single-stranded DNA oligonucleotides using fluorescence imaging technology. The sensor array was further characterized using a five-element lumped equivalent model. The 4 mm2 application-specific integrated circuit chip was designed and developed through 0.18 μm HV bipolar-CMOS-double diffused metal–oxide–silicon (DMOS) technology (BCD) to generate on-chip 20 V HV boosting and to track feedback frequency under a standard 1.8 V supply, with a total power consumption of 3.8 mW in a continuous mode. The measured results indicated a detection sensitivity of 7.943 × 10−3 μmol∙L−1∙Hz−1 over a concentration range of 1 to 100 μmol∙L−1. In conclusion, the label-free biosensing of DNA under dry conditions was successfully demonstrated using a microfabricated CMUT array with a 2 MHz frequency on CMOS electronics with an internal HV supplier. Moreover, ethylene–glycol alkanethiols successfully deposited self-assembled monolayers on aluminum electrodes, which has never been attempted thus far on CMUTs, to enhance the selectivity of bio-functionalization. The findings of this study indicate the possibility of full-on-chip DNA biosensing with CMUTs. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2095-8099 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2095809924002522; https://doaj.org/toc/2095-8099 |
| DOI: |
10.1016/j.eng.2023.12.015 |
| URL الوصول: |
https://doaj.org/article/0107707071aa4a36bc4ed4fef6584479 |
| رقم الانضمام: |
edsdoj.0107707071aa4a36bc4ed4fef6584479 |
| قاعدة البيانات: |
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