Academic Journal
Phosphorylation of cPLA2α at Ser505 Is Necessary for Its Translocation to PtdInsP2-Enriched Membranes
| العنوان: | Phosphorylation of cPLA2α at Ser505 Is Necessary for Its Translocation to PtdInsP2-Enriched Membranes |
|---|---|
| المؤلفون: | Javier Casas, Jesús Balsinde, María A. Balboa |
| المصدر: | Molecules, Vol 27, Iss 7, p 2347 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Organic chemistry |
| مصطلحات موضوعية: | cytosolic phospholipase A2α, arachidonic acid, membrane translocation, phosphorylation, phosphatidylinositol bisphosphate, Organic chemistry, QD241-441 |
| الوصف: | Group IVA cytosolic phospholipase A2α (cPLA2α) is a key enzyme in physiology and pathophysiology because it constitutes a rate-limiting step in the pathway for the generation of pro- and anti-inflammatory eicosanoid lipid mediators. cPLA2α activity is tightly regulated by multiple factors, including the intracellular Ca2+ concentration, phosphorylation reactions, and cellular phosphatidylinositol (4,5) bisphosphate levels (PtdInsP2). In the present work, we demonstrate that phosphorylation of the enzyme at Ser505 is an important step for the translocation of the enzyme to PtdInsP2–enriched membranes in human cells. Constructs of eGFP-cPLA2 mutated in Ser505 to Ala (S505A) exhibit a delayed translocation in response to elevated intracellular Ca2+, and also in response to increases in intracellular PtdInsP2 levels. Conversely, translocation of a phosphorylation mimic mutant (S505E) is fully observed in response to cellular increases in PtdInsP2 levels. Collectively, these results suggest that phosphorylation of cPLA2α at Ser505 is necessary for the enzyme to translocate to internal membranes and mobilize arachidonic acid for eicosanoid synthesis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 27072347 1420-3049 |
| Relation: | https://www.mdpi.com/1420-3049/27/7/2347; https://doaj.org/toc/1420-3049 |
| DOI: | 10.3390/molecules27072347 |
| URL الوصول: | https://doaj.org/article/00ec639b5d4f4272af520848065b464b |
| رقم الانضمام: | edsdoj.00ec639b5d4f4272af520848065b464b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 27072347 14203049 |
|---|---|
| DOI: | 10.3390/molecules27072347 |