Academic Journal
Cell-cycle inhibition by Helicobacter pylori L-asparaginase.
| العنوان: | Cell-cycle inhibition by Helicobacter pylori L-asparaginase. |
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| المؤلفون: | Claudia Scotti, Patrizia Sommi, Maria Valentina Pasquetto, Donata Cappelletti, Simona Stivala, Paola Mignosi, Monica Savio, Laurent Roberto Chiarelli, Giovanna Valentini, Victor M Bolanos-Garcia, Douglas Scott Merrell, Silvia Franchini, Maria Luisa Verona, Cristina Bolis, Enrico Solcia, Rachele Manca, Diego Franciotta, Andrea Casasco, Paola Filipazzi, Elisabetta Zardini, Vanio Vannini |
| المصدر: | PLoS ONE, Vol 5, Iss 11, p e13892 (2010) |
| بيانات النشر: | Public Library of Science (PLoS), 2010. |
| سنة النشر: | 2010 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Helicobacter pylori (H. pylori) is a major human pathogen causing chronic gastritis, peptic ulcer, gastric cancer, and mucosa-associated lymphoid tissue lymphoma. One of the mechanisms whereby it induces damage depends on its interference with proliferation of host tissues. We here describe the discovery of a novel bacterial factor able to inhibit the cell-cycle of exposed cells, both of gastric and non-gastric origin. An integrated approach was adopted to isolate and characterise the molecule from the bacterial culture filtrate produced in a protein-free medium: size-exclusion chromatography, non-reducing gel electrophoresis, mass spectrometry, mutant analysis, recombinant protein expression and enzymatic assays. L-asparaginase was identified as the factor responsible for cell-cycle inhibition of fibroblasts and gastric cell lines. Its effect on cell-cycle was confirmed by inhibitors, a knockout strain and the action of recombinant L-asparaginase on cell lines. Interference with cell-cycle in vitro depended on cell genotype and was related to the expression levels of the concurrent enzyme asparagine synthetase. Bacterial subcellular distribution of L-asparaginase was also analysed along with its immunogenicity. H. pylori L-asparaginase is a novel antigen that functions as a cell-cycle inhibitor of fibroblasts and gastric cell lines. We give evidence supporting a role in the pathogenesis of H. pylori-related diseases and discuss its potential diagnostic application. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC2976697?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0013892 |
| URL الوصول: | https://doaj.org/article/009b96e80e9f438c83cb24a07729194e |
| رقم الانضمام: | edsdoj.009b96e80e9f438c83cb24a07729194e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0013892 |