Academic Journal
Microcystin-LR induces lactate production disruption via altering the m6A modification in Sertoli cells
| العنوان: | Microcystin-LR induces lactate production disruption via altering the m6A modification in Sertoli cells |
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| المؤلفون: | Xiannan Meng, Wenju Li, Qingxuan Wu, Yue Gao, Ling Zhang |
| المصدر: | Ecotoxicology and Environmental Safety, Vol 263, Iss , Pp 115288- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Environmental pollution LCC:Environmental sciences |
| مصطلحات موضوعية: | MC-LR, Methyltransferase 3, Lactate dehydrogenase A, Igf2bp3, Testis, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350 |
| الوصف: | We have previously reported the toxicity of microcystin-LR (MC-LR) to the male reproductive system, which results in functional changes in mouse testes. In this study, mice were orally exposed to MC-LR at 1, 7.5, 15, or 30 μg/L daily for 180 days. We found an increase in germ cell apoptosis in the seminiferous tubules and low-quality sperm in the epididymis. A decrease in lactate dehydrogenase A (Ldha) expression in testes through high-throughput sequencing was observed. We validated that MC-LR disrupted lactate production in Sertoli cells by suppressing the expression of Ldha. Further studies identified that methyltransferase 3 (Mettl3) catalysed N6-methyladenosine (m6A) methylation of Ldha mRNA. Mettl3 was downregulated in Sertoli cells following exposure to MC-LR, decreasing m6A levels of Ldha. The stability of Ldha mRNA decreased when m6A levels of Ldha were inhibited. In conclusion, these results showed that MC-LR inhibits the expression of Ldha in an m6A-dependent manner, which might result in the apoptosis of spermatogenic cells and a decline in sperm quality. Our work provides a new perspective to understanding MC-LR-induced male infertility. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0147-6513 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0147651323007923; https://doaj.org/toc/0147-6513 |
| DOI: | 10.1016/j.ecoenv.2023.115288 |
| URL الوصول: | https://doaj.org/article/a00313df0af84f2d9a998b33777baff7 |
| رقم الانضمام: | edsdoj.00313df0af84f2d9a998b33777baff7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 01476513 |
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| DOI: | 10.1016/j.ecoenv.2023.115288 |