التفاصيل البيبلوغرافية
| العنوان: |
IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries |
| المؤلفون: |
Kin-Wai Tam, Cheuk-Yin Wong, Kenneth Lap-Kei Wu, Guy Lam, Xiaotong Liang, Wai-Ting Wong, Maximilian Tak-Sui Li, Wing-Yui Liu, Sa Cai, Graham Ka-Hon Shea, Daisy Kwok-Yan Shum, Ying-Shing Chan |
| المصدر: |
Cells, Vol 12, Iss 11, p 1479 (2023) |
| بيانات النشر: |
MDPI AG, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Cytology |
| مصطلحات موضوعية: |
Schwann cells, human bone marrow stromal cells, sensory neuron, remyelination, directed differentiation, sciatic nerve injury, Cytology, QH573-671 |
| الوصف: |
The in vitro derivation of Schwann cells from human bone marrow stromal cells (hBMSCs) opens avenues for autologous transplantation to achieve remyelination therapy for post-traumatic neural regeneration. Towards this end, we exploited human induced pluripotent stem-cell-derived sensory neurons to direct Schwann-cell-like cells derived from among the hBMSC-neurosphere cells into lineage-committed Schwann cells (hBMSC-dSCs). These cells were seeded into synthetic conduits for bridging critical gaps in a rat model of sciatic nerve injury. With improvement in gait by 12-week post-bridging, evoked signals were also detectable across the bridged nerve. Confocal microscopy revealed axially aligned axons in association with MBP-positive myelin layers across the bridge in contrast to null in non-seeded controls. Myelinating hBMSC-dSCs within the conduit were positive for both MBP and human nucleus marker HuN. We then implanted hBMSC-dSCs into the contused thoracic cord of rats. By 12-week post-implantation, significant improvement in hindlimb motor function was detectable if chondroitinase ABC was co-delivered to the injured site; such cord segments showed axons myelinated by hBMSC-dSCs. Results support translation into a protocol by which lineage-committed hBMSC-dSCs become available for motor function recovery after traumatic injury to both peripheral and central nervous systems. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2073-4409 |
| Relation: |
https://www.mdpi.com/2073-4409/12/11/1479; https://doaj.org/toc/2073-4409 |
| DOI: |
10.3390/cells12111479 |
| URL الوصول: |
https://doaj.org/article/000f0252e88541eea75e4619fd6c2a5b |
| رقم الانضمام: |
edsdoj.000f0252e88541eea75e4619fd6c2a5b |
| قاعدة البيانات: |
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