Dissertation/ Thesis
Cell viabilities and biodegradation rates of DNA/protamine complexes with two different molecular weights of DNA.
| العنوان: | Cell viabilities and biodegradation rates of DNA/protamine complexes with two different molecular weights of DNA. |
|---|---|
| المؤلفون: | Mori, Nana, Ohno, Jun, Sakagami, Ryuji, Hayakawa, Tohru, Fukushima, Tadao |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | DNA, protamine sulfate, DNA/protamine complex, DNA molecular weight, biodegradation rate, 3T3 Cells, Absorbable Implants, Animals, Biocompatible Materials, Cell Survival, Daunorubicin, Male, Materials Testing, Mice, Molecular Weight, Osteoblasts, Protamines, Rats, Rats, Sprague-Dawley, Solubility, Surface Properties, Tissue Engineering |
| Contents Note: | Cell viabilities and biodegradation rates of DNA/protamine complexes with two different molecular weights of DNA. Cell viabilities and biodegradation rates of DNA/protamine complexes with two different molecular weights of DNA. |
| نوع الوثيقة: | 博士論文 |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://ndlsearch.ndl.go.jp/books/R100000039-I9479197 |
| Degree: | 博士（歯学） -- 福岡歯科大学 |
| ملاحظات: | 収集根拠 : 博士論文（自動収集） 資料形態 : テキストデータ コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文 Two types of DNA/protamine complexes were prepared from protamine sulfate and 7000 base pair (bp) DNA or original DNA to investigate the effect of the molecular weight of DNA on zeta potential, cell viability, flowability, soft tissue response, and biodegradation rate. The 7000 bp DNA/protamine complex had a negative charge while the original DNA/protamine complex had a positive charge. The cell viabilities (90.4-106.8%) of these complexes were close to each other. The 7000 bp DNA/protamine complex became a softer dough than that of the original DNA/complex when both were kneaded with water. In vivo, the original DNA/protamine complex showed a milder tissue response. The original DNA/protamine complex almost disappeared 30 days after implantation. The 7000 bp DNA/complex disappeared approximately 2 weeks after implantation and areas where samples were implanted became empty. Thereafter, the empty space was gradually replaced by new soft tissues. The original DNA/protamine complex showed low intercalation and groove binding ratios of daunorubicin hydrochloride. Results indicate that high DNA condensation by cationic protamine protected the penetration of degradation enzymes into these complexes. It was found that a high molecular weight of DNA reduced the biodegradation rate and flowability. This study suggests that DNA/protamine complexes could be candidates for biomaterials that control biodegradation rates and flowability. J Biomed Mater Res B Appl Biomater. 2013 Jul;101(5):743-51. doi: 10.1002/jbm.b.32877. Epub 2013 Jan 29. Wiley Online Library |
| رقم الانضمام: | edsdlc.oai:ndlsearch.ndl.go.jp:R100000039.I9479197 |
| قاعدة البيانات: | National Diet Library Digital Collections - 国立国会図書館デジタルコレクション |
| الوصف غير متاح. |