Dissertation/ Thesis
Preconditioning exercise reduces brain damage and neuronal apoptosis through enhanced endogenous 14-3-3γ after focal brain ischemia in rats
| العنوان: | Preconditioning exercise reduces brain damage and neuronal apoptosis through enhanced endogenous 14-3-3γ after focal brain ischemia in rats 脳梗塞発症前運動は、ラットの局所脳虚血後、内在性14-3-3γの増強を介して脳損傷および神経細胞のアポトーシスを減少させる |
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| المؤلفون: | 大塚, 章太郎, 大塚, 章太郎 |
| المصدر: | Brain Structure and Function. 224(2) |
| بيانات النشر: | Springer, 2019. 鹿児島大学, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 14-3-3γ, HIF-1α, physical activity, ischemic tolerance, intrinsic factor |
| Contents Note: | 2023-08-05 再収集 (5コマ目) -- 2023-08-05 再収集 (6コマ目) -- 2023-08-05 再収集 (7コマ目) -- 2023-08-05 再収集 (8コマ目) |
| نوع الوثيقة: | 博士論文 |
| اللغة: | English |
| ملاحظات: | 14-3-3γ is an important early ischemia-inducible protective factor against ischemic cell death in cerebral cortical neurons. We investigated the anti-apoptosis mechanism of enhanced 14-3-3γ mediated by preconditioning exercise-induced brain ischemic tolerance after stroke. Rats were assigned to 4 groups: exercise and ischemia (Ex group), ischemia and no exercise (No-Ex group), exercise and no ischemia (Ex-only group), and no exercise and ischemia (control group). Rats were trained on a treadmill for 5 days a week for 3 weeks (running speed, 25 m/min; running duration, 30 min/day). After the exercise program, stroke was induced by left middle cerebral artery occlusion. The infarct volume, neurological deficits, and motor function, as well as expression levels of hypoxiainduced factor-1α (HIF-1α), 14-3-3γ, P2X7 receptors, p-β-catenin Ser37, Bax, and caspase 3 were evaluated by immunohistochemistry and western blotting. The expression of HIF-1α and 14-3-3γ significantly increased in neurons and astrocytes in the Ex-only group. HIF-1α was co-expressed with P2X7 receptor- and GFAP-positive astrocytes. After stroke, the Ex group had significantly reduced brain infarction. HIF-1α and 14-3-3γ significantly increased in the Ex group compared to the No-Ex group. In addition, p-β-catenin Ser37 significantly increased following elevated 14-3-3γ; in contrast, Bax and caspase 3 were significantly reduced in the Ex group. Our findings suggest that preconditioning exercise prior to ischemia induces neuron- and astrocyte-mediated brain ischemic tolerance through increased expression of HIF-1α and 14-3-3γ, which are intrinsic protective factors; the upregulated 14-3-3γ induced by preconditioning exercise reduces ischemic neuronal cell death through the 14-3-3γ/p-β-catenin Ser37/Bax/caspase 3 anti-apoptotic pathway. identifier:博士論文全文 identifier:博士論文要旨 identifier:最終試験結果の要旨 identifier:論文審査の要旨 |
| رقم الانضمام: | edsdlc.I002859787.00 |
| قاعدة البيانات: | National Diet Library Digital Collections - 国立国会図書館デジタルコレクション |
| الوصف غير متاح. |