التفاصيل البيبلوغرافية
| العنوان: |
Electrochemically reduced graphene and iridium oxide nanoparticles for inhibition-based angiotensin-converting enzyme inhibitor detection |
| المؤلفون: |
Kurbanoglu, Sevinc, Rivas, Lourdes, Ozkan, Sibel A., Merkoçi, Arben |
| سنة النشر: |
2017 |
| المجموعة: |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
| مصطلحات موضوعية: |
Captopril detection, Electrochemically reduced graphene oxide, Enzyme inhibition, Iridium oxide nanoparticles, Angiotensin-converting enzyme inhibitor, Enzyme biosensors |
| الوصف: |
In this work, a novel biosensor based on electrochemically reduced graphene oxide and iridium oxide nanoparticles for the detection of angiotensin-converting enzyme inhibitor drug, captopril, is presented. For the preparation of the biosensor, tyrosinase is immobilized onto screen printed electrode by using 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-Hydroxysuccinimide coupling reagents, in electrochemically reduced graphene oxide and iridium oxide nanoparticles matrix. Biosensor response is characterized towards catechol, in terms of graphene oxide concentration, number of cycles to reduce graphene oxide, volume of iridium oxide nanoparticles and tyrosinase solution. The designed biosensor is used to inhibit tyrosinase activity by Captopril, which is generally used to treat congestive heart failure. It is an angiotensin-converting enzyme inhibitor that operates via chelating copper at the active site of tyrosinase and thioquinone formation. The captopril detections using both inhibition ways are very sensitive with low limits of detection: 0.019 µM and 0.008 µM for chelating copper at the active site of tyrosinase and thioquinone formation, respectively. The proposed methods have been successfully applied in captopril determination in spiked human serum and pharmaceutical dosage forms with acceptable recovery values. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
18734235 |
| Relation: |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-260; Ministerio de EconomÃa y Competitividad SEV-2013-0295; Ministerio de EconomÃa y Competitividad MAT2014-52485-P; Biosensors & bioelectronics; Vol. 88 (February 2017), p. 122-129; https://ddd.uab.cat/record/225313; urn:10.1016/j.bios.2016.07.109; urn:oai:ddd.uab.cat:225313; urn:scopus_id:84994530408; urn:articleid:18734235v88p122; urn:icn2uab:6089387; urn:oai:egreta.uab.cat:publications/013a1cd1-a353-4af1-bf86-e4918433a3d1 |
| الاتاحة: |
https://ddd.uab.cat/record/225313 |
| Rights: |
open access ; Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. ; https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.FFEA409 |
| قاعدة البيانات: |
BASE |