Academic Journal
New Method for the Expansion of Highly Purified Human Regulatory Granzyme B-Expressing B Cells
| العنوان: | New Method for the Expansion of Highly Purified Human Regulatory Granzyme B-Expressing B Cells |
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| المؤلفون: | Chesneau, Mélanie, Le Mai, Hoa, Brouard, Sophie |
| المساهمون: | Centre de Recherche en Transplantation et Immunologie - Center for Research in Transplantation and Translational Immunology (U1064 Inserm - CR2TI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Team 4 : Deciphering organ immune regulation in inflammation and transplantation (DORI-t) (Team 4 - U1064 Inserm - CR2TI), Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), LabEX IGO Immunothérapie Grand Ouest, Nantes Université (Nantes Univ) |
| المصدر: | ISSN: 1064-3745. |
| بيانات النشر: | HAL CCSD Humana Press/Springer Imprint |
| سنة النشر: | 2021 |
| المجموعة: | Inserm: HAL (Institut national de la santé et de la recherche médicale) |
| مصطلحات موضوعية: | Breg, Expansion, Granzyme B, Regulation, T/B-cell coculture, MESH: Apoptosis / immunology, MESH: B-Lymphocytes / immunology, MESH: Humans, MESH: Lymphocyte Activation, MESH: T-Lymphocytes, Cytotoxic / immunology, MESH: B-Lymphocytes / metabolism, MESH: B-Lymphocytes, Regulatory / immunology, Regulatory / metabolism, MESH: Cell Culture Techniques / methods, MESH: Cell Proliferation, MESH: Granzymes / immunology, MESH: Granzymes / isolation & purification, MESH: Granzymes / metabolism, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Granzyme B (GZMB)-expressing B cells inhibit CD4+ T-lymphocyte proliferation in a contact- and GZMB-dependent manner, through degradation of TCR zeta or induction of T-cell apoptosis. This regulatory B-cell population is present in human healthy individuals and represents about 1% of circulating B cells. Their small proportion requires the development of expansion methods to enable their study and envision clinical applications. We describe here how to expand GZMB-expressing B cells to obtain more than 90% of highly purified GZMB+ B cells, and the protocol of B/T cells coculture for the evaluation of the suppressive function of the GZMB+ B-cell population. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/33479900; inserm-04574800; https://inserm.hal.science/inserm-04574800; PUBMED: 33479900 |
| DOI: | 10.1007/978-1-0716-1237-8_11 |
| الاتاحة: | https://inserm.hal.science/inserm-04574800 https://doi.org/10.1007/978-1-0716-1237-8_11 |
| رقم الانضمام: | edsbas.FFABA199 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/978-1-0716-1237-8_11 |
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