Academic Journal
PTEX helps efficiently traffic haemoglobinases to the food vacuole in Plasmodium falciparum
| العنوان: | PTEX helps efficiently traffic haemoglobinases to the food vacuole in Plasmodium falciparum |
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| المؤلفون: | Jonsdottir, Thorey K., Elsworth, Brendan, Cobbold, Simon, Gabriela, Mikha, Ploeger, Ellen, Parkyn Schneider, Molly, Charnaud, Sarah C., Dans, Madeline G., McConville, Malcolm, Bullen, Hayley E., Crabb, Brendan S., Gilson, Paul R. |
| المساهمون: | Dzikowski, Ron, National Health and Medical Research Council |
| المصدر: | PLOS Pathogens ; volume 19, issue 7, page e1011006 ; ISSN 1553-7374 |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2023 |
| المجموعة: | PLOS Publications (via CrossRef) |
| الوصف: | A key element of Plasmodium biology and pathogenesis is the trafficking of ~10% of the parasite proteome into the host red blood cell (RBC) it infects. To cross the parasite-encasing parasitophorous vacuole membrane, exported proteins utilise a channel-forming protein complex termed the Plasmodium translocon of exported proteins (PTEX). PTEX is obligatory for parasite survival, both in vitro and in vivo , suggesting that at least some exported proteins have essential metabolic functions. However, to date only one essential PTEX-dependent process, the new permeability pathways, has been described. To identify other essential PTEX-dependant proteins/processes, we conditionally knocked down the expression of one of its core components, PTEX150, and examined which pathways were affected. Surprisingly, the food vacuole mediated process of haemoglobin (Hb) digestion was substantially perturbed by PTEX150 knockdown. Using a range of transgenic parasite lines and approaches, we show that two major Hb proteases; falcipain 2a and plasmepsin II, interact with PTEX core components, implicating the translocon in the trafficking of Hb proteases. We propose a model where these proteases are translocated into the PV via PTEX in order to reach the cytostome, located at the parasite periphery, prior to food vacuole entry. This work offers a second mechanistic explanation for why PTEX function is essential for growth of the parasite within its host RBC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1371/journal.ppat.1011006 |
| الاتاحة: | http://dx.doi.org/10.1371/journal.ppat.1011006 https://dx.plos.org/10.1371/journal.ppat.1011006 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.FF6ECAC2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.ppat.1011006 |
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