Academic Journal
Effect of mono-(2-ethylhexyl) phthalate on human and mouse fetal testis: In vitro and in vivo approaches
| العنوان: | Effect of mono-(2-ethylhexyl) phthalate on human and mouse fetal testis: In vitro and in vivo approaches |
|---|---|
| المؤلفون: | Muczynski, Vincent, Cravedi, Jean Pierre, J. P., Lehraiki, Abdelali, Levacher, Christine, Moison, D., Lécureuil, Charlotte, Messiaen, S., Perdu, Elisabeth, Frydman, R., Habert, René, Rouiller-Fabre, V. |
| المساهمون: | Stabilité génétique, cellules souches et radiations (SCSR (U_967)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Stabilité génétique, Cellules Souches et Radiations (SCSR (U967)), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), ToxAlim (ToxAlim), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT), Service de gynécologie-obstétrique, médecine de la reproduction Béclère, Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Antoine Béclère Clamart, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP) |
| المصدر: | ISSN: 0041-008X. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2012 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | Phthalates Human Testis Germ cell Fetus, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction |
| الوصف: | International audience ; The present study was conducted to determine whether exposure to the mono-(2-ethylhexyl) phthalate (MEHP) represents a genuine threat to male human reproductive function. To this aim, we investigated the effects on human male fetal germ cells of a 10−5 M exposure. This dose is slightly above the mean concentrations found in human fetal cord blood samples by biomonitoring studies. The in vitro experimental approach was further validated for phthalate toxicity assessment by comparing the effects of in vitro and in vivo exposure in mouse testes. Human fetal testes were recovered during the first trimester (7-12 weeks) of gestation and cultured in the presence or not of 10−5 M MEHP for three days. Apoptosis was quantified by measuring the percentage of Caspase-3 positive germ cells. The concentration of phthalate reaching the fetal gonads was determined by radioactivity measurements, after incubations with 14C-MEHP. A 10−5 M exposure significantly increased the rate of apoptosis in human male fetal germ cells. The intratesticular MEHP concentration measured corresponded to the concentration added in vitro to the culture medium. Furthermore, a comparable effect on germ cell apoptosis in mouse fetal testes was induced both in vitro and in vivo. This study suggests that this 10−5 M exposure is sufficient to induce changes to the in vivo development of the human fetal male germ cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/22484159; PRODINRA: 178656; PUBMED: 22484159; WOS: 000304494600011 |
| DOI: | 10.1016/j.taap.2012.03.016 |
| الاتاحة: | https://hal.science/hal-00776675 https://hal.science/hal-00776675v1/document https://hal.science/hal-00776675v1/file/Muczynski_TAAP2012_1.pdf https://doi.org/10.1016/j.taap.2012.03.016 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.FF66916D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.taap.2012.03.016 |
|---|