Academic Journal
Vectorial Release of Hepatitis E Virus in Polarized Human Hepatocytes
| العنوان: | Vectorial Release of Hepatitis E Virus in Polarized Human Hepatocytes |
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| المؤلفون: | Capelli, Nicolas, Marion, Olivier, Dubois, Martine, Allart, Sophie, Bertrand-Michel, Justine, Lhomme, Sébastien, Abravanel, Florence, Izopet, Jacques, Chapuy-Regaud, Sabine |
| المساهمون: | Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Département de Néphrologie et Transplantation d'organes CHU Toulouse, Pôle Urologie - Néphrologie - Dialyse - Transplantations - Brûlés - Chirurgie plastique - Explorations fonctionnelles et physiologiques CHU Toulouse, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), MetaToul Lipidomics, MetaboHUB-MetaToul, MetaboHUB-Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut des Maladies Métaboliques et Casdiovasculaires (UPS/Inserm U1297 - I2MC), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | ISSN: 0022-538X. |
| بيانات النشر: | HAL CCSD American Society for Microbiology |
| سنة النشر: | 2019 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | TCID50, bile acid quantification, colocalization, hepatitis E virus, hepatocyte, lipid-associated particle, polarized cell, quasienveloped virus, MESH: Capsid Proteins / metabolism, MESH: Carcinoma, Hepatocellular / metabolism, MESH: Hepatitis E virus / physiology, MESH: Hepatocytes / metabolism, MESH: Hepatocytes / virology, MESH: Humans, MESH: Liver Neoplasms / metabolism, MESH: Serum / virology, MESH: Viral Proteins, MESH: Virus Release / physiology, MESH: Cell Culture Techniques y, MESH: Cell Line, MESH: Cell Polarity, MESH: Epithelial Cells / virology, MESH: Hep G2 Cells, MESH: Hepatitis E / virology, MESH: Hepatitis E virus / metabolism, MESH: Hepatitis E virus / pathogenicity, [SDV]Life Sciences [q-bio], [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: | International audience ; Hepatitis E virus (HEV) is a common cause of acute viral hepatitis worldwide. Most HEV infections are asymptomatic, but immunocompromised patients infected with HEV genotype 3 (HEV3), HEV4, or HEV7 may develop chronic infections. The HEV particles in stools are naked (nHEV), while those in the serum and culture supernatants (eHEV) are associated with lipids. Hepatocytes are polarized epithelial cells that have basolateral (oriented toward the blood) and apical (oriented toward the bile) exosomal pathways. We isolated a subclone, F2, from the human hepatocarcinoma cell line HepG2/C3A that grew as a polarized monolayer culture and had better HEV production than HepG2/C3A cells. F2 cells cultured on semipermeable collagen inserts and infected basolaterally with nHEV3 released 94.6% of virus particles apically, those infected with eHEV3 released 96.8% apically, and eHEV1-infected cells released 99.3% apically. Transcytosis was not involved. Density gradient centrifugation and NP-40 treatment showed that HEV particles released both apically and basolaterally were lipid associated. The apically released HEV3 and HEV1 particles were six and nine times more infectious than those released basolaterally, respectively. Confocal microscopy indicated that the open reading frame 2 (ORF2) capsid protein colocalized apically with ORF3 virus protein, the apical marker DPP4, and the recycling endosome GTPase Rab27a. The amounts of soluble glycosylated ORF2 secreted apically and basolaterally were similar. These polarized-hepatocyte data suggest that infectious HEV particles are mainly released into bile, while the small fraction released into blood could spread HEV throughout the host.IMPORTANCE Hepatitis E virus (HEV) in stools is naked, while that in culture supernatants and patients' blood is lipid associated. Its life cycle in hepatocytes, polarized cells with a basolateral side communicating with blood and an apical side connected with bile, is incompletely understood. We have developed a polarized ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/30463960; PUBMED: 30463960; PUBMEDCENTRAL: PMC6364016 |
| DOI: | 10.1128/JVI.01207-18 |
| الاتاحة: | https://ut3-toulouseinp.hal.science/hal-03608725 https://doi.org/10.1128/JVI.01207-18 |
| رقم الانضمام: | edsbas.FF522B4A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JVI.01207-18 |
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