Academic Journal
Modulation of murine dendritic cell function by adenine nucleotides and adenosine: involvement of the A(2B) receptor.
| العنوان: | Modulation of murine dendritic cell function by adenine nucleotides and adenosine: involvement of the A(2B) receptor. |
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| المؤلفون: | Ben Addi, Abduelhakem, Lefort, Anne, Hua, Xiaoyang, Libert, Frédérick, Communi, Didier, Ledent, Catherine, Macours, Pascale, Tilley, Stephen L, Boeynaems, Jean-Marie, Robaye, Bernard |
| المصدر: | European Journal of Immunology, 38 (6 |
| سنة النشر: | 2008 |
| المجموعة: | DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
| مصطلحات موضوعية: | Sciences bio-médicales et agricoles, Acetamides -- pharmacology, Adenine Nucleotides -- pharmacology, Adenosine -- pharmacology, Adenosine-5'-(N-ethylcarboxamide) -- pharmacology, Animals, Antigens, Surface -- metabolism, Arginase -- genetics, Arginase -- metabolism, Cyclic AMP -- metabolism, Cytokines -- metabolism, Dendritic Cells -- drug effects, Dendritic Cells -- metabolism, Gene Expression -- drug effects, Gene Expression Profiling, Interleukin-12 -- metabolism, Lipopolysaccharides -- pharmacology, Mice, Inbred C57BL, Knockout, Nitrites -- metabolism, Purines -- pharmacology, Receptor, Adenosine A2A -- agonists, Adenosine A2A -- antagonists & inhibitors, Adenosine A2A -- genetics, Adenosine A2B -- genetics, Adenosine A2B -- physiology, Receptors |
| الوصف: | Adenosine triphosphate has previously been shown to induce semi-mature human monocyte-derived dendritic cells (DC). These are characterized by the up-regulation of co-stimulatory molecules, the inhibition of IL-12 and the up-regulation of some genes involved in immune tolerance, such as thrombospondin-1 and indoleamine 2,3-dioxygenase. The actions of adenosine triphosphate are mediated by the P2Y(11) receptor; since there is no functional P2Y(11) gene in the murine genome, we investigated the action of adenine nucleotides on murine DC. Adenosine 5'-(3-thiotriphosphate) and adenosine inhibited the production of IL-12p70 by bone marrow-derived DC (BMDC). These inhibitions were relieved by 8-p-sulfophenyltheophylline, an adenosine receptor antagonist. The use of selective ligands and A(2B) (-/-) BMDC indicated the involvement of the A(2B) receptor. A microarray experiment, confirmed by quantitative PCR, showed that, in presence of LPS, 5'-(N-ethylcarboxamido) adenosine (NECA, the most potent A(2B) receptor agonist) regulated the expression of several genes: arginase I and II, thrombospondin-1 and vascular endothelial growth factor were up-regulated whereas CCL2 and CCL12 were down-regulated. We further showed that NECA, in combination with LPS, increased the arginase I enzymatic activity. In conclusion, the described actions of adenine nucleotides on BMDC are mediated by their degradation product, adenosine, acting on the A(2B) receptor, and will possibly lead to an impairment of Th1 response or tolerance. ; Journal Article ; Research Support, Non-U.S. Gov't ; FLWIN ; info:eu-repo/semantics/published |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 1 full-text file(s): application/pdf |
| اللغة: | English |
| Relation: | uri/info:doi/10.1002/eji.200737781; uri/info:pmid/18465770; uri/info:scp/49649091999; https://dipot.ulb.ac.be/dspace/bitstream/2013/50312/3/50312.pdf; http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50312 |
| الاتاحة: | http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/50312 https://dipot.ulb.ac.be/dspace/bitstream/2013/50312/3/50312.pdf |
| رقم الانضمام: | edsbas.FF119B92 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |