Academic Journal
Immobilised peroxidases from Asparagus acutifolius L. seeds for olive mill waste water treatment
| العنوان: | Immobilised peroxidases from Asparagus acutifolius L. seeds for olive mill waste water treatment |
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| المؤلفون: | Guida V, Niro E, Landi N, CHAMBERY, Angela, Parente A, Cantarella L, Cantarella M, DI MARO, Antimo |
| المساهمون: | Guida, V, Niro, E, Landi, N, Chambery, Angela, Parente, A, Cantarella, L, Cantarella, M, DI MARO, Antimo |
| سنة النشر: | 2014 |
| المجموعة: | Università degli Studi della Campania "Luigi Vanvitelli": CINECA IRIS V: |
| الوصف: | This work compares the main enzymatic parameters of a cationic peroxidase (AaP-1-4), purified and characterized from Asparagus acutifolius L. seeds (Mol. Biotechnol., 2014, 56, 738-746) and its immobilised form (Eup-AaP-1-4), on Eupergit® CM with Horseradish peroxidase. The optimum in the pH-activity profile was pH 4.0 and pH 3.0 for AaP-1-4 and Eup-AaP-1-4, respectively. Ca 2+ cation enhanced both enzymatic activities, however, when submitted to a temperature stress (120 min at 50 °C) Eup-AaP-1-4 lost only 20% activity while AaP-1-4 70%. Furthermore, AaP-1-4 was proved to be able to remove (poly)phenols in olive mill waste water (OMW), with hydrogen peroxide electron donor. The Eup-AaP-1-4 kinetic proprieties were investigated and the operational stability evaluated in a continuous stirred membrane bioreactor. AaP-1-4 appears to be a novel non-expensive source of peroxidases suitable for biotechnological applications in the environmental field for the removal of aqueous (poly)phenols produced from several industrial processes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000345656600032; volume:4; issue:106; firstpage:61482; lastpage:61490; numberofpages:9; journal:RSC ADVANCES; http://hdl.handle.net/11591/227444; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84912003066; http://pubs.rsc.org/en/journals/journalissues |
| DOI: | 10.1039/c4ra11310a |
| الاتاحة: | http://hdl.handle.net/11591/227444 https://doi.org/10.1039/c4ra11310a http://pubs.rsc.org/en/journals/journalissues |
| رقم الانضمام: | edsbas.FEF86E7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1039/c4ra11310a |
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