Academic Journal
The SPTLC3 subunit of serine palmitoyltransferase generates short chain sphingoid bases
| العنوان: | The SPTLC3 subunit of serine palmitoyltransferase generates short chain sphingoid bases |
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| المؤلفون: | Hornemann, Thorsten, Penno, A, Rütti, M F, Ernst, D, Kivrak-Pfiffner, Fatma, Rohrer, L, von Eckardstein, Arnold |
| المصدر: | Hornemann, Thorsten; Penno, A; Rütti, M F; Ernst, D; Kivrak-Pfiffner, Fatma; Rohrer, L; von Eckardstein, Arnold (2009). The SPTLC3 subunit of serine palmitoyltransferase generates short chain sphingoid bases. Journal of Biological Chemistry, 284(39):26322-26330. |
| بيانات النشر: | American Society for Biochemistry and Molecular Biology |
| سنة النشر: | 2009 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Institute of Clinical Chemistry, 610 Medicine & health, 540 Chemistry |
| الوصف: | The enzyme serine palmitoyltransferase (SPT) catalyzes the rate-limiting step in the de novo synthesis of sphingolipids. Previously the mammalian SPT was described as a heterodimer composed of two subunits, SPTLC1 and SPTLC2. Recently we identified a novel third SPT subunit (SPTLC3). SPTLC3 shows about 68% identity to SPTLC2 and also includes a pyridoxal phosphate consensus motif. Here we report that the overexpression of SPTLC3 in HEK293 cells leads to the formation of two new sphingoid base metabolites, namely C(16)-sphinganine and C(16)-sphingosine. SPTLC3-expressing cells have higher in vitro SPT activities with lauryl- and myristoyl-CoA than SPTLC2-expressing cells, and SPTLC3 mRNA expression levels correlate closely with the C(16)-sphinganine synthesis rates in various human and murine cell lines. Approximately 15% of the total sphingolipids in human plasma contain a C(16) backbone and are found in the high density and low density but not the very low density lipoprotein fraction. In conclusion, we show that the SPTLC3 subunit generates C(16)-sphingoid bases and that sphingolipids with a C(16) backbone constitute a significant proportion of human plasma sphingolipids. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0021-9258 |
| Relation: | https://www.zora.uzh.ch/id/eprint/24506/1/Hornemann_2009.pdf; info:pmid/19648650; urn:issn:0021-9258 |
| DOI: | 10.1074/jbc.M109.023192 |
| الاتاحة: | https://www.zora.uzh.ch/id/eprint/24506/ https://www.zora.uzh.ch/id/eprint/24506/1/Hornemann_2009.pdf https://doi.org/10.1074/jbc.M109.023192 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.FE898D51 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00219258 |
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| DOI: | 10.1074/jbc.M109.023192 |