Academic Journal
Isolation and pharmacological characterization of two functional splice variants of corticotropin-releasing factor type 2 receptor from Tupaia belangeri
| العنوان: | Isolation and pharmacological characterization of two functional splice variants of corticotropin-releasing factor type 2 receptor from Tupaia belangeri |
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| المؤلفون: | Palchaudhuri, M. R., Hauger, R. L., Wille, S., Fuchs, E., Dautzenberg, F. M. |
| سنة النشر: | 1999 |
| المجموعة: | Georg-August-Universität Göttingen: GoeScholar |
| الوصف: | From brain, heart and muscle tissue of the tree shrew (Tupaia belangeri), a higher order mammal, cDNA clones were isolated that encoded two functional splice variants of the corticotropin-releasing factor (CRF) type 2 receptor (CRF-R2). The first, full-length splice variant, amplified from brain and heart tissue, encoded a CRF receptor protein that is 410 amino acids in length and approximately 96% homologous to human CRF-R2alpha. The second, full-length splice variant, derived from skeletal muscle tissue, encoded a 437-amino acid CRF receptor protein that is approximately 92% homologous to human CRF-R2beta. Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) amplifications and RNase protection analyses, showed that tree shrew CRF-R2alpha (tCRF-R2alpha) and tree shrew CRF-R2beta (tCRF-R2beta) were coexpressed in brain tissue but not in heart and skeletal muscle tissue. Finally, human embryonic kidney 293 (HEK293) cells stably transfected with tCRF-R2alpha and tCRF-R2beta were used to demonstrate that the CRF analogs urocortin and sauvagine bind with significantly greater affinity (21- to 140-fold) to these two CRF-R2 splice variants than do human/rat and ovine CRF analogs. In keeping with these results of our CRF binding studies, EC50 values were substantially lower for urocortin-and sauvagine-stimulated than for h/rCRF-and oCRF-stimulated cyclic AMP accumulation in HEK293 cells stably transfected with tCRF-R2alpha or tCRF-R2beta cDNAs. The tree shrew therefore constitutes an important animal model in which to investigate the role of CRF receptor subtypes in the stress response. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0953-8194 |
| Relation: | Deutsches Primatenzentrum; https://resolver.sub.uni-goettingen.de/purl?gro-2/116289 |
| DOI: | 10.1046/j.1365-2826.1999.00348.x |
| الاتاحة: | https://resolver.sub.uni-goettingen.de/purl?gro-2/116289 https://doi.org/10.1046/j.1365-2826.1999.00348.x |
| رقم الانضمام: | edsbas.FE58462B |
| قاعدة البيانات: | BASE |
| تدمد: | 09538194 |
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| DOI: | 10.1046/j.1365-2826.1999.00348.x |