Academic Journal
Menstrual blood-derived mesenchymal stromal cells: impact of preconditioning on the cargo of extracellular vesicles as potential therapeutics
| العنوان: | Menstrual blood-derived mesenchymal stromal cells: impact of preconditioning on the cargo of extracellular vesicles as potential therapeutics |
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| المؤلفون: | de Pedro, María Ángeles, López, Esther, González-Nuño, Francisco Manuel, Pulido, María, Álvarez, Verónica, Marchena, Ana María, Preußer, Christian, Szymański, Witold, Pogge von Strandmann, Elke, Graumann, Johannes, Sánchez-Margallo, Francisco Miguel, Casado, Javier G., Gómez-Serrano, María |
| المساهمون: | Deutsche Forschungsgemeinschaft, Instituto de Salud Carlos III, Junta de Extremadura, Philipps-Universität Marburg |
| المصدر: | Stem Cell Research & Therapy ; volume 14, issue 1 ; ISSN 1757-6512 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| الوصف: | Background Mesenchymal stromal cells (MSCs) have been shown to exert their therapeutic effects through the secretion of broad spectrum of paracrine factors, including extracellular vesicles (EVs). Accordingly, EVs are being pursued as a promising alternative to cell-based therapies. Menstrual blood-derived stromal cells (MenSCs) are a type of MSC that, due to their immunomodulatory and regenerative properties, have emerged as an innovative source. Additionally, new strategies of cell priming may potentially alter the concentration and cargo of released EVs, leading to modification of their biological properties. In this study, we aimed to characterize the EVs released by MenSCs and compare their therapeutic potential under three different preconditioning conditions (proinflammatory stimuli, physioxia, and acute hypoxia). Methods MenSCs were isolated from five healthy women. Following culturing to 80% confluence, MenSCs were exposed to different priming conditions: basal (21% O 2 ), proinflammatory stimuli (IFNγ and TNFα, 21% O 2 ), physioxia (1–2% O 2 ), and acute hypoxia (< 1% O 2 ) for 48–72 h. Conditioned media from MenSCs was collected after 48 h and EVs were isolated by a combination of ultra-filtration and differential centrifugation. An extensive characterization ranging from nano-flow cytometry (nFC) to quantitative high-throughput shotgun proteomics was performed. Bioinformatics analyses were used to derive hypotheses on their biological properties. Results No differences in the morphology, size, or number of EVs released were detected between priming conditions. The proteome analysis associated with basal MenSC-EVs prominently revealed their immunomodulatory and regenerative capabilities. Furthermore, quantitative proteomic analysis of differentially produced MenSC-EVs provided sufficient evidence for the utility of the differential preconditioning in purpose-tailoring EVs for their therapeutic application: proinflammatory priming enhanced the anti-inflammatory, regenerative and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s13287-023-03413-5 |
| DOI: | 10.1186/s13287-023-03413-5.pdf |
| DOI: | 10.1186/s13287-023-03413-5/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s13287-023-03413-5 https://link.springer.com/content/pdf/10.1186/s13287-023-03413-5.pdf https://link.springer.com/article/10.1186/s13287-023-03413-5/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.FD5B77A3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13287-023-03413-5 |
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