Academic Journal
In vivo imaging of adenovirus transduction and enhanced therapeutic efficacy of combination therapy with conditionally replicating adenovirus and adenovirus-p27
| العنوان: | In vivo imaging of adenovirus transduction and enhanced therapeutic efficacy of combination therapy with conditionally replicating adenovirus and adenovirus-p27 |
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| المؤلفون: | Lee, Choon-Taek, Lee, Yoon-Jin, Kwon, Sung-Youn, Lee, Jaeho, Kim, Kwang Il, Park, Kyung-Ho, Kang, Joo Hyun, Yoo, Chul-Gyu, Kim, Young Whan, Han, Sung Koo, Chung, June-Key, Shim, Young-Soo, Curiel, David T, Carbone, David P |
| المساهمون: | 이춘택, 이윤진, 권성연, 이재호, 김광일, 박경호, 강주현, 유철규, 김영환, 한성구, 정준기, 심영수 |
| بيانات النشر: | American Association for Cancer Research |
| سنة النشر: | 2006 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | Adenoviridae/genetics/*physiology, Animals, Cyclin-Dependent Kinase Inhibitor p27/biosynthesis/*genetics, Gene Expression, Gene Therapy/*methods, Humans, Luciferases/biosynthesis/genetics, Luminescent Measurements, Lung Neoplasms/genetics/metabolism/*therapy/virology, Mice, Transduction, Genetic, Virus Replication, Xenograft Model Antitumor Assays |
| الوصف: | Gene therapy is hampered by poor gene transfer to the tumor mass. We previously proposed a combination adenoviral gene therapy containing a conditionally replicating adenovirus (CRAD) expressing mutant E1 (delta24RGD) and a replication-defective E1-deleted adenovirus to enhance the efficiency of gene transfer. Mutant E1 expressed by delta24RGD enables the replication of replication-defective adenoviruses in tumors when cancer cells are co-infected with both viruses. In this study, gene transfer rates in xenografts tumors were monitored by bioluminescence in cells infected with the replication-defective adenovirus-luciferase (ad-luc). Tumor masses treated with CRAD + ad-luc showed dramatically stronger and more prolonged luciferase expression than ad-luc-treated tumors and this expression spread through the entire tumor mass without significant systemic spread. Transduction with CRAD + replication-defective adenovirus-p27 increased the expression of p27 by 24-fold versus transduction with ad-p27 alone. Treatment of a lung cancer cell line and of established lung cancer xenografts with CRAD + adenovirus-p27 also induced stronger growth suppression than treatment with either virus alone. These findings confirm the selective replication of E1-deleted adenovirus containing a therapeutic gene due to the presence of mutant E1 produced by delta24RGD in tumors. Moreover, this replication increased the therapeutic gene transfer rate and enhanced its antitumor effects. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Cancer Res. 2006 Jan 1;66(1):372-7.; 0008-5472 (Print); http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16397251; https://hdl.handle.net/10371/28252 |
| DOI: | 10.1158/0008-5472.CAN-05-1515 |
| الاتاحة: | https://hdl.handle.net/10371/28252 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16397251 https://doi.org/10.1158/0008-5472.CAN-05-1515 |
| رقم الانضمام: | edsbas.FD4CDBC8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1158/0008-5472.CAN-05-1515 |
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