التفاصيل البيبلوغرافية
| العنوان: |
Comparison of Osteoconductive Ability of Two Types of Cholesterol-Bearing Pullulan (CHP) Nanogel-Hydrogels Impregnated with BMP-2 and RANKL-Binding Peptide: Bone Histomorphometric Study in a Murine Calvarial Defect Model |
| المؤلفون: |
Cangyou Xie, Fatma Rashed, Yosuke Sasaki, Masud Khan, Jia Qi, Yuri Kubo, Yoshiro Matsumoto, Shinichi Sawada, Yoshihiro Sasaki, Takashi Ono, Tohru Ikeda, Kazunari Akiyoshi, Kazuhiro Aoki |
| المصدر: |
International Journal of Molecular Sciences; Volume 24; Issue 11; Pages: 9751 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2023 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
bone regeneration, scaffold material, bone morphogenetic protein (BMP)-2, receptor activator of NF-κB ligand (RANKL)-binding peptide, cholesterol-bearing pullulan (CHP) nanogels |
| جغرافية الموضوع: |
agris |
| الوصف: |
The receptor activator of NF-κB ligand (RANKL)-binding peptide is known to accelerate bone morphogenetic protein (BMP)-2-induced bone formation. Cholesterol-bearing pullulan (CHP)-OA nanogel-crosslinked PEG gel (CHP-OA nanogel-hydrogel) was shown to release the RANKL-binding peptide sustainably; however, an appropriate scaffold for peptide-accelerated bone formation is not determined yet. This study compares the osteoconductivity of CHP-OA hydrogel and another CHP nanogel, CHP-A nanogel-crosslinked PEG gel (CHP-A nanogel–hydrogel), in the bone formation induced by BMP-2 and the peptide. A calvarial defect model was performed in 5-week-old male mice, and scaffolds were placed in the defect. In vivo μCT was performed every week. Radiological and histological analyses after 4 weeks of scaffold placement revealed that the calcified bone area and the bone formation activity at the defect site in the CHP-OA hydrogel were significantly lower than those in the CHP-A hydrogel when the scaffolds were impregnated with both BMP-2 and the RANKL-binding peptide. The amount of induced bone was similar in both CHP-A and CHP-OA hydrogels when impregnated with BMP-2 alone. In conclusion, CHP-A hydrogel could be an appropriate scaffold compared to the CHP-OA hydrogel when the local bone formation was induced by the combination of RANKL-binding peptide and BMP-2, but not by BMP-2 alone. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Materials Science; https://dx.doi.org/10.3390/ijms24119751 |
| DOI: |
10.3390/ijms24119751 |
| الاتاحة: |
https://doi.org/10.3390/ijms24119751 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.FD46FDBC |
| قاعدة البيانات: |
BASE |