التفاصيل البيبلوغرافية
| العنوان: |
S4 Fig - |
| المؤلفون: |
Murshed H. Sarkar (11740364), Ryoji Yagi (4032284), Yukihiro Endo (10049504), Ryo Koyama-Nasu (104436), Yangsong Wang (10049507), Ichita Hasegawa (11740367), Toshihiro Ito (197968), Ilkka S. Junttila (11740370), Jinfang Zhu (736381), Motoko Y. Kimura (6662465), Toshinori Nakayama (46666) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Cell Biology, Molecular Biology, Immunology, Developmental Biology, Cancer, Hematology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, ifn &# 947, growth factor independent, 1 transcriptional repressor, div >< p, th2 cells results, th2 cell proliferation, type ii response, th2 cells, results demonstrate, immune response, known cytokine, key molecule, fully understood, also known, actively promotes |
| الوصف: |
(A) A schematic illustration of the experimental protocol. Naïve CD4 T cells were isolated from Gfi1 fl/fl CreER T2 (CD45.2), Gfi1 +/+ CreER T2 (CD45.2) and CD45.1 congenic mice and cultured under Th2 conditions. Gfi1 fl/fl CreER T2 (KO) or Gfi1 +/+ CreER T2 (WT) Th2 cells were mixed together with CD45.1 Wt Th2 cells, labelled with CFSE and further cultured with indicated cytokines (i.e., IL-2 and IFNγ) in the presence of 4-OHT for the indicated number of days. (B) GFI-1 mRNA expression relative to Hprt on cells after treatment of 4-OHT for 1 day. (C) The cytokine production of Th2 cells generated from the indicated mice as described in (A). Numbers indicate the percentage in each quadrant. Data are representative of three independent experiments. (D and E) A schematic illustration of the experimental protocol using retroviral infection. Naïve CD4 T cells were isolated from C57BL/6 mice and cultured under Th2 conditions for 5 days. In vitro -differentiated Th2 cells were then labelled with CFSE, infected with the GFI1-Thy1.1-retrovirus (RV) or empty-Thy1.1-RV and further cultured with IL-2 in the presence or absence of IFNγ. The MFI of CFSE was measured on day 3 or 4 by flow cytometry (D). The infection efficiency of Control-Thy1.1 RV and GFI1-Thy1.1 RV (E). (PDF) |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/S4_Fig_-/17062637 |
| DOI: |
10.1371/journal.pone.0260204.s004 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0260204.s004 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.FD3A3B31 |
| قاعدة البيانات: |
BASE |