التفاصيل البيبلوغرافية
| العنوان: |
Transcriptomic responses in human intestinal cells (Caco-2, HIEC-6) exposed to dietary nanoparticles ... |
| المؤلفون: |
Basu, Niladri, Xu, Ke, Mittal, Krittika, Rulli, Samuel, Jakubowski, Jennifer, George, Saji |
| بيانات النشر: |
Borealis |
| سنة النشر: |
2022 |
| المجموعة: |
DataCite Metadata Store (German National Library of Science and Technology) |
| مصطلحات موضوعية: |
Agricultural Sciences, Earth and Environmental Sciences, Medicine, Health and Life Sciences, toxicogenomics, genomics, New Approach Methods NAMs, alternative to animal models, high throughput, toxicology, food safety |
| الوصف: |
Use of nanoparticles (NPs) in the food industry raises health concerns particularly in the gastrointestinal system. Assessing dietary NPs remains challenged due the vast number of products and the resource-intensive nature of traditional toxicity testing. Recent advancements in high-throughput transcriptomics, coupled with benchmark dose (BMD) analysis are poised to modernize chemical safety assessments. The objective of this study was to derive transcriptomic point of departure (tPOD) values for common dietary NPs through dose-response analysis of 3’ RNA-sequencing data. Two intestinal cell lines (Caco-2, HIEC-6) were exposed to 9 forms of Ag, SiO2, and TiO2 (including Food Grade and Non-Food Grade particles, as well as Nanoparticles and Microparticles), and expression of L1000 landmark genes was characterized using the QIAseq UPX 3’ targeted RNA panel kit catalog number 333041 / CSHS-10608Z-990. Each transcript in each well of the RT plate was labeled with a unique molecular index (UMI) for each cDNA ... : There are two files uploaded. #1 - All RNAseq data are in one spreadsheet with 4 tabs (Xu et al. RNASeq Data)) #2 - The data were next analyzed for transcriptomics points of departure using www.FastBMD.ca, and these data are provided as 8 spreadsheet files. ... |
| نوع الوثيقة: |
dataset |
| وصف الملف: |
text/tab-separated-values |
| اللغة: |
unknown |
| DOI: |
10.5683/sp3/1bbfce |
| DOI: |
10.5683/SP3/1BBFCE |
| الاتاحة: |
https://dx.doi.org/10.5683/sp3/1bbfce
https://borealisdata.ca/citation?persistentId=doi:10.5683/SP3/1BBFCE |
| Rights: |
Creative Commons Zero v1.0 Universal ; info:eu-repo/semantics/openAccess ; https://creativecommons.org/publicdomain/zero/1.0/legalcode ; cc0-1.0 |
| رقم الانضمام: |
edsbas.FD0CEFC9 |
| قاعدة البيانات: |
BASE |