التفاصيل البيبلوغرافية
| العنوان: |
The PAH1‐encoded Phosphatidate Phosphatase Plays a Role in Lipogenesis in the Oleaginous Yeast Yarrowia lipolytica |
| المؤلفون: |
Hardman, Derell, Ukey, Rahul, Fakas, Stylianos |
| المساهمون: |
U.S. Department of Agriculture |
| المصدر: |
The FASEB Journal ; volume 32, issue S1 ; ISSN 0892-6638 1530-6860 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2018 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
The PAH1 gene encodes for phosphatidate phosphatase (PAP), the enzyme that catalyzes the dephosphorylation of phosphatidic acid to yield diacylglycerol and P i . PAP activity is essential for the biosynthesis of the storage lipid triacylglycerol (TAG) in several eukaryotes (e.g. Saccharomyces cerevisiae ). In Yarrowia lipolytica , TAG accumulates during lipogenesis and is responsible for the increase in cellular lipid content under these conditions. We hypothesized that under lipogenic conditions, lack of the Y. lipolytica PAH1 homolog (i.e. pah1 Δ) should result in a reduction of lipid content and diminished TAG levels. To examine this hypothesis, we cultivated the Y. lipolytica wild type and pah1 Δ strains in a lipogenic (8% glucose) medium, and analyzed their growth and lipid profiles. The results showed that the pah1 Δ mutant exhibited increased growth but a lower lipid content in comparison to the wild type. However, the pah1 Δ mutant was able to synthesize TAG, although to a lesser extent than the wild type. These results indicate that the Y. lipolytica PAH1 homolog contributes to, but is not essential for, TAG biosynthesis during lipogenesis. Support or Funding Information This research was supported by Evans‐Allen Grant no.1005735 and Capacity Building Grants Program Grant no.1007503 from the USDA National Institute of Food and Agriculture, as well as the Strengthening Historically Black Graduate Institutions Program under Title III of the Higher Education Act of 1965. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal . |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1096/fasebj.2018.32.1_supplement.539.20 |
| الاتاحة: |
http://dx.doi.org/10.1096/fasebj.2018.32.1_supplement.539.20 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#am ; http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: |
edsbas.FCFA9000 |
| قاعدة البيانات: |
BASE |