Academic Journal
Characterization and alteration of product specificity of Beijerinckia indica subsp. indica β-fructosyltransferase
| العنوان: | Characterization and alteration of product specificity of Beijerinckia indica subsp. indica β-fructosyltransferase |
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| المؤلفون: | Li, Ding, Miyasaka, Yuki, Kubota, Arisa, Kozono, Takuma, Kitano, Yoshikazu, Sasaki, Nobumitsu, Fujii, Tadashi, Tochio, Takumi, Kadota, Yoshihiro, Nishikawa, Atsushi, Tonozuka, Takashi |
| المساهمون: | Japan Society for the Promotion of Science, Program on Open Innovation Platform with Enterprises, Research Institute and Academia, Japan Science and Technology Agency, Photon Factory Advisory Committee |
| المصدر: | Bioscience, Biotechnology, and Biochemistry ; volume 87, issue 9, page 981-990 ; ISSN 1347-6947 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2023 |
| الوصف: | The trisaccharide 1-kestose, a major constituent of fructooligosaccharide, has strong prebiotic effects. We used high-performance liquid chromatography and 1H nuclear magnetic resonance spectroscopy to show that BiBftA, a β-fructosyltransferase belonging to glycoside hydrolase family 68, from Beijerinckia indica subsp. indica catalyzes transfructosylation of sucrose to produce mostly 1-kestose and levan polysaccharides. We substituted His395 and Phe473 in BiBftA with Arg and Tyr, respectively, and analyzed the reactions of the mutant enzymes with 180 g/L sucrose. The ratio of the molar concentrations of glucose and 1-kestose in the reaction mixture with wild-type BiBftA was 100:8.1, whereas that in the reaction mixture with the variant H395R/F473Y was 100:45.5, indicating that H395R/F473Y predominantly accumulated 1-kestose from sucrose. The X-ray crystal structure of H395R/F473Y suggests that its catalytic pocket is unfavorable for binding of sucrose while favorable for transfructosylation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/bbb/zbad074 |
| DOI: | 10.1093/bbb/zbad074/50695560/zbad074.pdf |
| الاتاحة: | https://doi.org/10.1093/bbb/zbad074 https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbad074/50695560/zbad074.pdf https://academic.oup.com/bbb/article-pdf/87/9/981/51232573/zbad074.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.FCBEA26C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/bbb/zbad074 |
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