التفاصيل البيبلوغرافية
| العنوان: |
open access www.bioinformation.net Database Volume 8(1) The capsicum transcriptome DB: a “hot ” tool for |
| المؤلفون: |
Elsa Góngora-castillo, Rubén Fajardo-jaime, Araceli Fernández-cortes, Alba E. Jofre, Edmundo Lozoya-gloria, Octavio Martínez, Neftalí Ochoa-alejo, Rafael Rivera |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/c1/85/Bioinformation_2012_Jan_6_8(1)_43-47.tar.gz |
| سنة النشر: |
2011 |
| المجموعة: |
CiteSeerX |
| الوصف: |
Chili pepper (Capsicum annuum) is an economically important crop with no available public genome sequence. We describe a genomic resource to facilitate Capsicum annuum research. A collection of Expressed Sequence Tags (ESTs) derived from five C. annuum organs (root, stem, leaf, flower and fruit) were sequenced using the Sanger method and multiple leaf transcriptomes were deeply sampled using with GS-pyrosequencing. A hybrid assembly of 1,324,516 raw reads yielded 32,314 high quality contigs as validated by coverage and identity analysis with existing pepper sequences. Overall, 75.5 % of the contigs had significant sequence similarity to entries in nucleic acid and protein databases; 23 % of the sequences have not been previously reported for C. annuum and expand sequence resources for this species. A MySQL database and a user-friendly Web interface were constructed with search-tools that permit queries of the ESTs including sequence, functional annotation, Gene Ontology classification, metabolic pathways, and assembly information. The Capsicum Transcriptome DB is free available from |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/zip |
| اللغة: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.271.6924 |
| الاتاحة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.271.6924 |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.FC7BC078 |
| قاعدة البيانات: |
BASE |